2. Academic Validation
  3. Anti-neuroinflammatory effect of aurantiamide acetate from the marine fungus Aspergillus sp. SF-5921: inhibition of NF-κB and MAPK pathways in lipopolysaccharide-induced mouse BV2 microglial cells

Anti-neuroinflammatory effect of aurantiamide acetate from the marine fungus Aspergillus sp. SF-5921: inhibition of NF-κB and MAPK pathways in lipopolysaccharide-induced mouse BV2 microglial cells

  • Int Immunopharmacol. 2014 Dec;23(2):568-74. doi: 10.1016/j.intimp.2014.10.006.
Chi-Su Yoon 1,  Dong-Cheol Kim 1,  Dong-Sung Lee 2,  Kyoung-Su Kim 1,  Wonmin Ko 3,  Jae Hak Sohn 4,  Joung Han Yim 5,  Youn-Chul Kim 6,  Hyuncheol Oh 7
Affiliations

Affiliations

  • 1 Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea; Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea.
  • 2 Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 570-749, Republic of Korea.
  • 3 Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea.
  • 4 College of Medical and Life Sciences, Silla University, Busan 617-736, Republic of Korea.
  • 5 Korea Polar Research Institute, KORDI, 7-50 Songdo-dong, Yeonsu-gu, Incheon 406-840, Republic of Korea.
  • 6 Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea. Electronic address: yckim@wku.ac.kr.
  • 7 Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, 570-749, Republic of Korea. Electronic address: hoh@wku.ac.kr.
PMID: 25448500 DOI: 10.1016/j.intimp.2014.10.006
Abstract

In the course of a search for anti-neuroinflammatory metabolites from marine fungi, aurantiamide acetate (1) was isolated from marine-derived Aspergillus sp. as an anti-neuroinflammatory component. Compound 1 dose-dependently inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in BV2 microglial cells. It also attenuated inducible NO Synthase (iNOS), cyclooxygenase-2 (COX-2), and other pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In a further study designed to elucidate the mechanism of its anti-neuroinflammatory effect, compound 1 was shown to block the activation of nuclear factor-kappa B (NF-κB) in lipopolysaccharide (LPS)-induced BV2 microglial cells by inhibiting the phosphorylation of the inhibitor kappa B-α (IκB)-α. In addition, compound 1 decreased the phosphorylation levels of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases (MAPKs). These results suggest that compound 1 has an anti-neuroinflammatory effect on LPS stimulation through its inhibition of the NF-κB, JNK and p38 pathways.

Keywords

Anti-neuroinflammation; Aspergillus sp.; Aurantiamide acetate; BV2 microglial cells; Marine fungus.

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