2. Academic Validation
  3. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing

Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing

  • Nat Cell Biol. 2015 Jan;17(1):31-43. doi: 10.1038/ncb3076.
Julia K Pagan 1,  Antonio Marzio 1,  Mathew J K Jones 2,  Anita Saraf 3,  Prasad V Jallepalli 2,  Laurence Florens 3,  Michael P Washburn 4,  Michele Pagano 5
Affiliations

Affiliations

  • 1 Department of Pathology, Laura and Isaac Perlmutter Cancer Center, New York University School of Medicine, New York 10065, USA.
  • 2 Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue New York 10065, USA.
  • 3 The Stowers Institute of Medical Research, 1000 East 50th Street, Kansas City Missouri 64110, USA.
  • 4 1] The Stowers Institute of Medical Research, 1000 East 50th Street, Kansas City Missouri 64110, USA [2] Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City Kansas 66160, USA.
  • 5 1] Department of Pathology, Laura and Isaac Perlmutter Cancer Center, New York University School of Medicine, New York 10065, USA [2] Howard Hughes Medical Institute, 522 First Avenue New York 10016, USA.
PMID: 25503564 DOI: 10.1038/ncb3076
Abstract

An intercentrosomal linker keeps a cell's two centrosomes joined together until it is dissolved at the onset of mitosis. A second connection keeps daughter centrioles engaged to their mothers until they lose their orthogonal arrangement at the end of mitosis. Centriole disengagement is required to license centrioles for duplication. We show that the intercentrosomal linker protein Cep68 is degraded in prometaphase through the SCF(βTrCP) (Skp1-Cul1-F-box protein) ubiquitin Ligase complex. Cep68 degradation is initiated by PLK1 phosphorylation of Cep68 on Ser 332, allowing recognition by βTrCP. We also found that Cep68 forms a complex with Cep215 (also known as Cdk5Rap2) and PCNT (also known as pericentrin), two PCM (pericentriolar material) proteins involved in centriole engagement. Cep68 and PCNT bind to different pools of Cep215. We propose that Cep68 degradation allows Cep215 removal from the peripheral PCM preventing centriole separation following disengagement, whereas PCNT cleavage mediates Cep215 removal from the core of the PCM to inhibit centriole disengagement and duplication.

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