2. Academic Validation
  3. CDy6, a photostable probe for long-term real-time visualization of mitosis and proliferating cells

CDy6, a photostable probe for long-term real-time visualization of mitosis and proliferating cells

  • Chem Biol. 2015 Feb 19;22(2):299-307. doi: 10.1016/j.chembiol.2014.11.018.
Yun-Mi Jeong 1,  Zhai Duanting 2,  Holger Hennig 3,  Animesh Samanta 1,  Bikram Keshari Agrawalla 2,  Mark-Anthony Bray 3,  Anne E Carpenter 3,  Young-Tae Chang 4
Affiliations

Affiliations

  • 1 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore.
  • 2 Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.
  • 3 Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
  • 4 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore; Department of Chemistry, National University of Singapore, Singapore 117543, Singapore. Electronic address: chmcyt@nus.edu.sg.
PMID: 25601075 DOI: 10.1016/j.chembiol.2014.11.018
Abstract

Long-term real-time visualization of lysosomal dynamics has been challenging at the onset of mitosis due to the lack of fluorescent probes enabling convenient imaging of dividing cells. We developed a long-term real-time photostable mitotic or proliferating marker, CDy6, a BODIPY-derived compound of designation yellow 6, which labels lysosome. In long-term real-time, CDy6 displayed a sharp increase in intensity and change in localization in mitosis, improved photostability, and decreased toxicity compared with other widely used lysosomal and DNA markers, and the ability to label cells in mouse xenograft models. Therefore, CDy6 may open new possibilities to target and trace lysosomal contents during mitosis and to monitor cell proliferation, which can further our knowledge of the basic underlying biological mechanisms in the management of Cancer.

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