2. Academic Validation
  3. Preparative isolation and purification of lignans from Justicia procumbens using high-speed counter-current chromatography in stepwise elution mode

Preparative isolation and purification of lignans from Justicia procumbens using high-speed counter-current chromatography in stepwise elution mode

  • Molecules. 2015 Apr 20;20(4):7048-58. doi: 10.3390/molecules20047048.
Peijuan Zhou 1,  Qijun Luo 2,  Lijian Ding 3 4,  Fang Fang 5,  Ye Yuan 6,  Juanjuan Chen 7,  Jinrong Zhang 8,  Haixiao Jin 9,  Shan He 10
Affiliations

Affiliations

  • 1 Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. zhoupeijuanhaha@163.com.
  • 2 Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. luoqijun@nbu.edu.cn.
  • 3 Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. huahua20062008@126.com.
  • 4 College of Pharmacy, Jinan University, Guangzhou 510632, China. huahua20062008@126.com.
  • 5 Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. 15888107865@163.com.
  • 6 Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. 23yuanye@163.com.
  • 7 Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. chenjuanjuan@nbu.edu.cn.
  • 8 Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. zhangjinrong@nbu.edu.cn.
  • 9 Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. jinhaixiao@nbu.edu.cn.
  • 10 Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China. heshan@nbu.edu.cn.
PMID: 25903362 DOI: 10.3390/molecules20047048
Abstract

Lignans, which are recognized as main constituents in Justicia procumbens, have attracted considerable attention due to their pharmacological activities, including antitumor, anti-hepatitic, cytotoxic, anti-microbial, and anti-virus properties. Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four Lignans (justicidin B (1), justicidin A (2), 6'-hydroxyjusticidin C (3) and lignan J1 (4)) from J. procumbens using stepwise elution with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at (1.3:1:1.3:1, v/v) and (2.5:1:2.5:1, v/v). The preparative HSCCC separation was performed on 300 mg of crude sample yielding compounds 1 (19.7 mg), 2 (9.86 mg), 3 (11.26 mg), and 4 (2.54 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these compounds were identified by MS, 1H-NMR and 13C-NMR. This is the first report on the application of HSCCC to the efficient separation of Lignans from J. procumbens.

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