2. Academic Validation
  3. Effects of resveratrol and methoxyamine on the radiosensitivity of iododeoxyuridine in U87MG glioblastoma cell line

Effects of resveratrol and methoxyamine on the radiosensitivity of iododeoxyuridine in U87MG glioblastoma cell line

  • Exp Biol Med (Maywood). 2016 Jun;241(11):1229-36. doi: 10.1177/1535370215622583.
Samideh Khoei 1,  Mohsen Shoja 2,  Ahmad Mostaar 3,  Fariborz Faeghi 2
Affiliations

Affiliations

  • 1 Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran 1449614525, Iran Razi Drug Research Centre, Iran University of Medical Sciences, Tehran 1449614525, Iran Khoei.s@iums.ac.ir.
  • 2 Radiology Technology Department, School of Paramedicine, Shahid Beheshti University of Medical Sciences, Tehran 1449614525, Iran.
  • 3 Medical Physics and Medical Engineering Department, Shahid Beheshti University of Medical Sciences, Tehran 1449614525, Iran.
PMID: 26748400 DOI: 10.1177/1535370215622583
Abstract

The purpose of this study was to evaluate the combination effect of resveratrol and methoxyamine on radiosensitivity of iododeoxyuridine in spheroid culture of U87MG glioblastoma cell line using colony formation and alkaline comet assays. Spheroids on day-20 with 350 µm diameters were treated with 20 µM resveratrol and/or 6 mM methoxyamine and/or 1 µM iododeoxyuridine for one volume doubling time (67 h), and then irradiated with 2 Gy gamma-radiation ((60)Co) in different groups. After treatment, viability of the cells, colony forming ability and DNA damages were obtained by blue dye exclusion, colony formation and alkaline comet assay, respectively. Our results showed that methoxyamine and resveratrol could significantly reduce colony number and induce the DNA damages of glioblastoma spheroid cells treated with iododeoxyuridine in combination with gamma-rays. Therefore, methoxyamine as base excision repair inhibitor and resveratrol as hypoxia inducible factor 1-alpha inhibitor in combination with iododeoxyuridine as radiosensitizer enhanced the radiosensitization of glioblastoma spheroid cells.

Keywords

Resveratrol; gamma-radiation; glioblastoma; iododeoxyuridine; methoxyamine.

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