1. Academic Validation
  2. Recombinant soluble gp130 protein reduces DEN-induced primary hepatocellular carcinoma in mice

Recombinant soluble gp130 protein reduces DEN-induced primary hepatocellular carcinoma in mice

  • Sci Rep. 2016 Apr 15;6:24397. doi: 10.1038/srep24397.
Jing Hong 1 Hang Wang 1 Guoying Shen 1 Da Lin 1 Yanxue Lin 1 Nanhui Ye 1 Yashan Guo 1 Qiaoling Li 1 Nanhui Ye 1 Chengjun Deng 1 Chun Meng 1
Affiliations

Affiliation

  • 1 Institute of Pharmaceutical Biotechnology and Engineering, College of Biological Science and Biotechnology, Fuzhou University, Fuzhou, Fujian, 350002, China.
Abstract

IL-6 (interleukin 6) plays an important role in the development and growth of hepatocellular carcinoma (HCC) via both classic signaling and trans-signaling pathways. Soluble gp130 (sgp130) is known to be a natural inhibitor of the trans-signaling pathway. In the present study, our goal was to investigate whether recombinant sgp130 could suppress the initiation and progression of HCC in mouse models. Our results demonstrate that sgp130 induced an Apoptosis of HepG2 cells and inhibited the clonogenicity of HepG2 in vitro. Moreover, the IL-6 trans-signaling pathway is significantly suppressed by sgp130 as reflected by the decrease in the level of STAT3 phosphorylation and Other inflammatory factors both in vitro and in vivo. In the DEN-induced HCC mouse model, intravenous injection of sgp130 attenuated hepatic fibrosis at 16 weeks and reduced the initiation and progression of primary HCC at 36 weeks. Furthermore, our results also demonstrate that intravenous administration of sgp130 significantly suppressed the growth and metastasis of xenograft human HCC in NOD/SCID mice.

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