2. Academic Validation
  3. Poly-l-lysine/heparin multilayer coatings prevent blood protein adsorption

Poly-l-lysine/heparin multilayer coatings prevent blood protein adsorption

  • J Colloid Interface Sci. 2017 Jan 1:485:288-295. doi: 10.1016/j.jcis.2016.09.046.
Alejandro Barrantes 1,  Jonas Wengenroth 2,  Thomas Arnebrant 3,  Håvard J Haugen 4
Affiliations

Affiliations

  • 1 Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. Box 1109 Blindern, 0317 Oslo, Norway; Biomedical Sciences, Health and Society, Malmö University, 20560 Malmö, Sweden. Electronic address: a.b.bautista@odont.uio.no.
  • 2 Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. Box 1109 Blindern, 0317 Oslo, Norway. Electronic address: jonas.wengenroth@odont.uio.no.
  • 3 Biomedical Sciences, Health and Society, Malmö University, 20560 Malmö, Sweden. Electronic address: thomas.arnebrant@mah.se.
  • 4 Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. Box 1109 Blindern, 0317 Oslo, Norway. Electronic address: h.j.haugen@odont.uio.no.
PMID: 27684786 DOI: 10.1016/j.jcis.2016.09.046
Abstract

The adsorption of blood proteins, serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (FGN), onto model SiO2 planar surfaces coated with poly-l-lysine/heparin multilayers (PLL/HEP) has been investigated by means of ellipsometry and quartz crystal microbalance with dissipation. Aiming at the development of low fouling coatings, this study has been focused on the effects that the number of layers and the type of polyelectrolyte present on the topmost layer have on the adsorption of these proteins. The three proteins interact with PLL-ended coatings whereas HEP-ended coatings prevent the adsorption of both BSA and IgG and induce a decrease in the adsorbed amount of FGN, down to 0.4mg/m2 for three bilayers, as the number of PLL/HEP bilayers increases. These results suggest that heparin-ended multilayers prevent protein adsorption, which is an indicative of good blood compatibility. As a consequence we propose that PLL/HEP coatings could be used for the development of vascular medical devices.

Keywords

Ellipsometry; Heparin coating; Layer by layer; Protein adsorption; QCM-D.

Figures
Products