2. Academic Validation
  3. Determination of Relative Response Factors of Cefazolin Impurities by Quantitative NMR

Determination of Relative Response Factors of Cefazolin Impurities by Quantitative NMR

  • AAPS PharmSciTech. 2017 Aug;18(6):1895-1900. doi: 10.1208/s12249-016-0654-4.
Shuyu Liu 1,  Shangchen Yao 2,  Hailing Zhang 1,  Changqin Hu 3
Affiliations

Affiliations

  • 1 College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
  • 2 National Institutes for Food and Drug Control, No.2 Tiantan Xili, Dongcheng, Beijing, 100050, China.
  • 3 National Institutes for Food and Drug Control, No.2 Tiantan Xili, Dongcheng, Beijing, 100050, China. hucq@nicpbp.org.cn.
PMID: 27853995 DOI: 10.1208/s12249-016-0654-4
Abstract

The relative response factors (RRFs) of ten cefazolin impurities were determined by quantitative nuclear magnetic resonance (qNMR) and high-performance liquid chromatography (HPLC) equipped with an ultraviolet (UV) detector. The purities of these ten cefazolin impurities were successfully measured by qNMR for the purpose of RRFs determination by HPLC. The RRF values and their uncertainties determined by the two approaches are comparable. While the qNMR approach is effective and makes it easier to determine the RRFs for impurities, it also has the advantage of allowing the universal detection of protons without the limitations of common mass detectors. The use of qNMR provides a reliable and universal method for the RRF determination of impurities.

Keywords

HPLC; RRF; qNMR; uncertainty.

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