2. Academic Validation
  3. Amplification of Adipogenic Commitment by VSTM2A

Amplification of Adipogenic Commitment by VSTM2A

  • Cell Rep. 2017 Jan 3;18(1):93-106. doi: 10.1016/j.celrep.2016.12.015.
Blandine Secco 1,  Étienne Camiré 1,  Marc-Antoine Brière 1,  Alexandre Caron 1,  Armande Billong 1,  Yves Gélinas 1,  Anne-Marie Lemay 1,  Kevin M Tharp 2,  Peter L Lee 3,  Stéphane Gobeil 4,  Jean V Guimond 5,  Natacha Patey 6,  David A Guertin 3,  Andreas Stahl 2,  Élie Haddad 7,  David Marsolais 1,  Yohan Bossé 1,  Kivanc Birsoy 8,  Mathieu Laplante 9
Affiliations

Affiliations

  • 1 Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec (CRIUCPQ), Université Laval, Faculté de médecine, 2725 Chemin Ste-Foy, QC G1V 4G5, Canada.
  • 2 Program for Metabolic Biology, Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • 3 University of Massachusetts Medical School, Program in Molecular Medicine, Worcester, MA 01605, USA.
  • 4 Centre hospitalier universitaire de Québec (CHU de Québec), Université Laval, Faculté de médecine, 2705 Boulevard Laurier, QC G1V 4G2, Canada.
  • 5 CIUSSS du Centre-Sud-de-l'ile-de-Montréal, CLSC des Faubourgs, 66 rue Sainte-Catherine Est, Montréal, QC H2X 1K6, Canada.
  • 6 Centre Hospitalier Universitaire de Sainte-Justine (CHU de Sainte-Justine), Faculté de Médecine, Département de pathologie et biologie cellulaire, Université de Montréal, 3175 Chemin Côte Ste-Catherine, Montréal, QC H3T 1C5, Canada.
  • 7 Centre Hospitalier Universitaire de Sainte-Justine (CHU de Sainte-Justine), Faculté de Médecine, Département de pédiatrie et Département de microbiologie, infectiologie et immunologie, Université de Montréal, 3175 Chemin Côte Ste-Catherine, Montréal, QC H3T 1C5, Canada.
  • 8 Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
  • 9 Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec (CRIUCPQ), Université Laval, Faculté de médecine, 2725 Chemin Ste-Foy, QC G1V 4G5, Canada. Electronic address: mathieu.laplante@criucpq.ulaval.ca.
PMID: 28052263 DOI: 10.1016/j.celrep.2016.12.015
Abstract

Despite progress in our comprehension of the mechanisms regulating adipose tissue development, the nature of the factors that functionally characterize adipose precursors is still elusive. Defining the early steps regulating adipocyte development is needed for the generation of tools to control adipose tissue size and function. Here, we report the discovery of V-set and transmembrane domain containing 2A (VSTM2A) as a protein expressed and secreted by committed preadipocytes. VSTM2A expression is elevated in the early phases of adipogenesis in vitro and adipose tissue development in vivo. We show that VSTM2A-producing cells associate with the vasculature and express the common surface markers of adipocyte progenitors. Overexpression of VSTM2A induces adipogenesis, whereas its depletion impairs this process. VSTM2A controls preadipocyte determination at least in part by modulating BMP signaling and PPARγ2 activation. We propose a model in which VSTM2A is produced to preserve and amplify the adipogenic capability of adipose precursors.

Keywords

3T3-L1; adipogenesis; adipogenic commitment; adipose tissue; cell differentiation; cell signaling; obesity; preadipocyte.

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