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  3. Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling

Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling

  • Beilstein J Nanotechnol. 2016 Dec 2;7:1905-1917. doi: 10.3762/bjnano.7.182.
Nadezhda M Zholobak 1,  Anton L Popov 2,  Alexander B Shcherbakov 1,  Nelly R Popova 2,  Mykhailo M Guzyk 3,  Valeriy P Antonovich 4,  Alla V Yegorova 4,  Yuliya V Scrypynets 4,  Inna I Leonenko 4,  Alexander Ye Baranchikov 5,  Vladimir K Ivanov 6
Affiliations

Affiliations

  • 1 Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv 03680, Ukraine.
  • 2 Institute of Theoretical and Experimental Biophysics, Pushchino 142290, Russia.
  • 3 Palladin Institute of Biochemistry NAS of Ukraine, Kyiv 01601, Ukraine.
  • 4 Bogatsky Physico-Chemical Institute, National Academy of Sciences of Ukraine, Odessa 65080, Ukraine.
  • 5 Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow 119991, Russia.
  • 6 Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow 119991, Russia; National Research Tomsk State University, Tomsk 634050, Russia.
PMID: 28144539 DOI: 10.3762/bjnano.7.182
Abstract

Luminescent organic dots (O-dots) were synthesized via a one-pot, solvent-free thermolysis of citric acid in urea melt. The influence of the ratio of the precursors and the duration of the process on the properties of the O-dots was established and a mechanism of their formation was hypothesized. The multicolour luminescence tunability and toxicity of synthesized O-dots were extensively studied. The possible applications of O-dots for alive/fixed cell staining and labelling of layer-by-layer polyelectrolyte microcapsules were evaluated.

Keywords

cell culture; citric acid; layer-by-layer (LbL)-microcapsules; luminescence; organic dots (O-dots); staining; toxicity.

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