2. Academic Validation
  3. Copper sulfate pentahydrate reduced epithelial cytotoxicity induced by lipopolysaccharide from enterogenic bacteria

Copper sulfate pentahydrate reduced epithelial cytotoxicity induced by lipopolysaccharide from enterogenic bacteria

  • Biomed Pharmacother. 2017 May:89:454-461. doi: 10.1016/j.biopha.2017.02.060.
Adel Feyzi 1,  Aref Delkhosh 1,  Hamid Tayefi Nasrabadi 2,  Omid Cheraghi 3,  Mansour Khakpour 4,  Mazyar Barekati-Mowahed 5,  Sina Soltani 6,  Seyede Momeneh Mohammadi 7,  Masoumeh Kazemi 7,  Mehdi Hassanpour 8,  Aysa Rezabakhsh 9,  Nasrin Maleki-Dizaji 10,  Reza Rahbarghazi 11,  Reza Namdarian 12
Affiliations

Affiliations

  • 1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
  • 2 Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 3 Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 4 Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
  • 5 Department of Physiology & Biophysics, Case Western Reserve University- School of Medicine, Cleveland, OH, United States.
  • 6 Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 7 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 8 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 9 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 10 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: malekins@tbzmed.ac.ir.
  • 11 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Rahbarghazir@tbzmed.ac.ir.
  • 12 Department of Poultry disease, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
PMID: 28249246 DOI: 10.1016/j.biopha.2017.02.060
Abstract

The over usage of multiple Antibiotics contributes to the emergence of a whole range of antibiotic-resistant strains of bacteria causing enterogenic infections in poultry science. Therefore, finding an appropriate alternative natural substance carrying an Antibacterial capacity would be immensely beneficial. It has been previously discovered that the different types of cupric salts, especially copper sulfate pentahydrate (CuSO4·5H2O), to carry a potent bactericidal capacity. We investigated the neutralizing effect of CuSO4·5H2O (6.25μg/ml) on the Reactive Oxygen Species generation, and expression of MyD88, an essential adaptor protein of Toll-like Receptor, and NF-κB in three intestinal epithelial cell lines exposed to 50ng/ml lipopolysaccharide. In order to find the optimal cupric sulfate concentration without enteritis-inducing toxicity, broiler chickens were initially fed with water containing 0.4, 0.5, and 1mg/l during a period of 4days. After determination of appropriate dosage, two broiler chickens and turkey flocks with enteritis were fed with cupric compound for 4days. We found that cupric sulfate can lessen the cytotoxic effect of lipopolysaccharide by reducing the Reactive Oxygen Species content (p<0.05). Additionally, the expression of MyD88 and NF-κB was remarkably down-regulated in the presence of lipopolysaccharide and cupric sulfate. The copper sulfate in doses lower than 0.4mg/ml expressed no cytotoxic effect on the liver, kidney, and the intestinal tract while a concentration of 0.5 and 1mg/ml contributed to a moderate to severe tissue injuries. Pearson Chi-Square analysis revealed the copper cation significantly diminished the rate of mortality during 4-day feeding of broiler chicken and turkey with enteritis (p=0.000). Thus, the results briefed above all confirm the potent anti-bactericidal feature of cupric sulfate during the course of enteritis.

Keywords

Broiler chickens; Copper sulfate pentahydrate; Intestinal epithelial cells; Lipopolysaccharide; Toll-like receptor signaling.

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