2. Academic Validation
  3. Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection

Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection

  • Int J Nanomedicine. 2017 Apr 20:12:3295-3302. doi: 10.2147/IJN.S128556.
Quan-Quan Zhuang 1,  Zhi-Hang Lin 1,  Yan-Cheng Jiang 1,  Hao-Hua Deng 2,  Shao-Bin He 1 3,  Li-Ting Su 4,  Xiao-Qiong Shi 2,  Wei Chen 2
Affiliations

Affiliations

  • 1 Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou.
  • 2 Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou.
  • 3 Department of Pharmacy, Quanzhou Infectious Disease Hospital.
  • 4 Department of Pharmaceutical Analysis, Quanzhou Medical College, Quanzhou, People's Republic of China.
PMID: 28458547 DOI: 10.2147/IJN.S128556
Abstract

Dendrite-like cobalt selenide nanostructures were synthesized from cobalt and selenium powder precursors by a solvothermal method in anhydrous ethylenediamine. The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H2O2. A spectrophotometric method for uric acid (UA) determination was developed based on the nanocrystalline cobalt selenide-catalyzed coupling reaction between N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and 4-aminoantipyrine (4-AAP) in the presence of H2O2. Under optimum conditions, the absorbance was proportional to the concentration of UA over the range of 2.0-40 μM with a detection limit of 0.5 μM. The applicability of the proposed method has been validated by determination of UA in human serum samples with satisfactory results.

Keywords

cobalt selenide; enzyme mimics; human serum; peroxidase-like activity; uric acid.

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