2. Academic Validation
  3. Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property

Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property

  • Molecules. 2017 May 19;22(5):844. doi: 10.3390/molecules22050844.
Chang-Hao Cui 1,  Da Jung Kim 2,  Suk-Chae Jung 3,  Sun-Chang Kim 4 5 6,  Wan-Taek Im 7
Affiliations

Affiliations

  • 1 Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. seldoms@163.com.
  • 2 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. scjung@kaist.ac.kr.
  • 3 Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. kimdj1404@kaist.ac.kr.
  • 4 Intelligent Synthetic Biology Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. sunkim@kaist.ac.kr.
  • 5 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. sunkim@kaist.ac.kr.
  • 6 KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. sunkim@kaist.ac.kr.
  • 7 Department of Biological Sciences, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 456-749, Korea. wandra@hknu.ac.kr.
PMID: 28534845 DOI: 10.3390/molecules22050844
Abstract

Minor ginsenosides, such as compound K, Rg₃(S), which can be produced by deglycosylation of ginsenosides Rb₁, showed strong anti-cancer effects. However, the Anticancer effects of gypenoside LXXV, which is one of the deglycosylated shapes of ginsenoside Rb₁, is still unknown due to the rarity of its content in Plants. Here, we cloned and characterized a novel ginsenoside-transforming β-glucosidase (BglG167b) derived from Microbacterium sp. Gsoil 167 which can efficiently hydrolyze gypenoside XVII into gypenoside LXXV, and applied it to the production of gypenoside LXXV at the gram-scale with high specificity. In addition, the anti-cancer activity of gypenoside LXXV was investigated against three Cancer cell lines (HeLa, B16, and MDA-MB231) in vitro. Gypenoside LXXV significantly reduced cell viability, displaying an enhanced anti-cancer effect compared to gypenoside XVII and Rb₁. Taken together, this enzymatic method would be useful in the preparation of gypenoside LXXV for the functional food and pharmaceutical industries.

Keywords

Microbacterium sp. Gsoil 167; biotransformation; deglycosylation; ginsenoside; gypenoside LXXV; gypenoside XVII.

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