2. Academic Validation
  3. Stephanine from Stephania venosa (Blume) Spreng Showed Effective Antiplasmodial and Anticancer Activities, the Latter by Inducing Apoptosis through the Reverse of Mitotic Exit

Stephanine from Stephania venosa (Blume) Spreng Showed Effective Antiplasmodial and Anticancer Activities, the Latter by Inducing Apoptosis through the Reverse of Mitotic Exit

  • Phytother Res. 2017 Sep;31(9):1357-1368. doi: 10.1002/ptr.5861.
Phuong Mai Le 1,  Vandana Srivastava 2,  Thanh Tam Nguyen 3,  Bruno Pradines 4 5 6,  Marylin Madamet 4 5 6,  Joel Mosnier 4 5 6,  Thi Thuy Trinh 3,  Hoyun Lee 2
Affiliations

Affiliations

  • 1 Measurement Science and Standards, National Research Council Canada, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada.
  • 2 Health Science North Research Institute, 41 Ramsey Lake Road, Sudbury, ON, P3E 5J1, Canada.
  • 3 Institute of Chemistry and Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi, Vietnam.
  • 4 Unité Parasitologie et Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France.
  • 5 Aix Marseille Université, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, IRD 198, Inserm 1095, Institut hospitalo-universitaire (IHU) Méditerranée Infection, Marseille, France.
  • 6 Centre National de Référence du Paludisme, Marseille, France.
PMID: 28703314 DOI: 10.1002/ptr.5861
Abstract

Extracts from the tubers of Stephania venosa (Blum) Spreng growing in Vietnam significantly inhibited cell proliferation against a number of Cancer cells including HeLa, MDA-MB231 and MCF-7 cells. A bioassay-guided fractionation led to the isolation of four aporphine and one tetrahydroprotoberberine alkaloids: dehydrocrebanine 1, tetrahydropalmatine 2, stephanine 3, crebanine 4 and O-methylbulbocapnine 5. The characterization of these compounds was based on MS, NMR and published data. A study by structure-bioactivity relationship on these isolates showed that stephanine is the most active compound. Cell biological studies showed that stephanine induces the reverse of mitotic exit, eventually leading to cell death by Apoptosis. This data suggests that stephanine has a unique mode of cell-killing activity against Cancer cells, which is seldom observed with known synthetic compounds. In addition to its Anticancer property, our data from an in vitro study showed that S. venosa also possesses effective antiplasmodial activity and stephanine was also the most interesting compound but is the most cytotoxic with the lowest selectivity index. Copyright © 2017 Her Majesty the Queen in Right of Canada Phytotherapy Research StartCopTextCopyright © 2017 John Wiley & Sons, Ltd.

Keywords

Stephania venosa; alkaloids; antiplasmodial; apoptosis; reverse of mitotic exit.

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