2. Academic Validation
  3. Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway

Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway

  • Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):800-807. doi: 10.1093/abbs/gmx072.
Nuramina Mamat 1 2 3,  Jun Dou 1 2,  Xueying Lu 1 2,  Aiden Eblimit 4,  Aisa Haji Akber 1 2
Affiliations

Affiliations

  • 1 The Key Laboratory of Plant Resources and Chemistry of Arid Zone, The Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
  • 2 State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Urumqi 830011, China.
  • 3 University of the Chinese Academy of Sciences, Beijing 100039, China.
  • 4 Department of Molecular and Human Genetics, Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA.
PMID: 28910976 DOI: 10.1093/abbs/gmx072
Abstract

Isochlorogenic acid A, also called 3,5-dicaffeoylquinic acid (3,5-diCQA), is a widespread phenolic compound in the plant. Recent studies have shown that it has antioxidant and anti-inflammatory activity. In addition, oxidative stress and inflammation induced by solar ultraviolet radiation is a very significant reason for skin depigmentation. Therefore, in this study, we evaluated the effect of 3,5-diCQA on B16 cells and explored its molecular mechanism. Results showed that 3,5-diCQA upregulated intracellular melanin production in a time- and dose-dependent manner. Tyrosinase (TYR) activity was also increased after treatment with 3,5-diCQA in a dose-dependent manner. Expressions of TYR, TYR-related protein1, TYR-related protein2, and microphthalmia-associated transcription factor were upregulated in a dose-dependent manner after 48 h of treatment with 3,5-diCQA. Results also showed that 3,5-diCQA promoted the phosphorylation of Akt at Thr308 and glycogen synthase kinase-3β at Ser 9. Moreover, 3,5-diCQA increased the content of β-catenin in cell cytoplasm and nucleus by reducing the content of phosphorylated β-catenin (p-β-catenin). All these results suggest that 3,5-diCQA may mediate the acceleration of melanin synthesis by the β-catenin signal pathway.

Keywords

isochlorogenic acid A; melanogenesis; β-catenin; β-catenin signal pathway.

Figures
Products