2. Academic Validation
  3. Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis

Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis

  • Front Biosci (Schol Ed). 2018 Jan 1;10(2):248-261. doi: 10.2741/s513.
Rengasamy Balakrishnan 1,  Namasivayam Elangovan 2,  Thangavel Mohankumar 1,  Jegadeesan Nataraj 3,  Thamilarasan Manivasagam 3,  Arokiasamy Justin Thenmozhi 4,  Mohamed Musthafa Essa 5,  Mohammed Akbar 6,  Mohammed Abdul Sattar Khan 7
Affiliations

Affiliations

  • 1 Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai nagar, Salem-636011, Tamilnadu, India.
  • 2 Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai nagar, Salem-636011, Tamilnadu, India, elangovannn@gmail.com.
  • 3 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai nagar-608 002, Tamilnadu, India.
  • 4 Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu 608002, India.
  • 5 Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman, Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman, Food and Brain Research Foundation, Chennai, Tamil Nadu 600094, India.
  • 6 NIAA, National Institute of Health, Rockville, MD, USA.
  • 7 Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospitals for Children, Boston, MA 02114, USA.
PMID: 28930531 DOI: 10.2741/s513
Abstract

The present study was carried out to investigate the neuroprotective effects of isolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction, oxidative stress and Apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and Apoptosis through the regulation of P13K/Akt/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.

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