2. Academic Validation
  3. Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells

Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells

  • Biochem Biophys Rep. 2016 Jul 17:7:287-294. doi: 10.1016/j.bbrep.2016.07.012.
L Pasquet 1 2,  E Bellard 1 2,  M P Rols 1 2,  M Golzio 1 2,  J Teissie 1 2
Affiliations

Affiliations

  • 1 Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, BP64182, 205 route de Narbonne, F-31077 Toulouse, France.
  • 2 Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, UPS, IPBS, F-31077 Toulouse, France.
PMID: 28955917 DOI: 10.1016/j.bbrep.2016.07.012
Abstract

Electric field mediated gene transfer is facing a problem in expression yield due to the poor transfer across the nuclear envelope. Trans-cyclohexane-1,2-diol (TCHD) was shown to significantly increase chemically mediated transfection by collapsing the permeability barrier of the nuclear pore complex. We indeed observed a significant increase in expression by electrotransfer when cells are treated post pulse by a low non toxic concentration of TCHD. This was obtained for different pulsing conditions, cell strains and plasmid constructs. An interesting improvement in cell viability can be obtained. This can significantly enhance the non-viral gene electrical delivery.

Keywords

Electroporation; Electrotransfer; Trans-cyclohexane-1,2-diol (TCHD); Viability.

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