1. Academic Validation
  2. Kudinoside-D, a triterpenoid saponin derived from Ilex kudingcha suppresses adipogenesis through modulation of the AMPK pathway in 3T3-L1 adipocytes

Kudinoside-D, a triterpenoid saponin derived from Ilex kudingcha suppresses adipogenesis through modulation of the AMPK pathway in 3T3-L1 adipocytes

  • Fitoterapia. 2018 Mar;125:208-216. doi: 10.1016/j.fitote.2017.11.018.
Yanyun Che 1 Qiuhong Wang 2 Ruyue Xiao 3 Jiayu Zhang 4 Yaqiong Zhang 1 Wen Gu 1 Gaoxiong Rao 1 Changfu Wang 5 Haixue Kuang 6
Affiliations

Affiliations

  • 1 Engineering Laboratory for National Healthcare Theories and Products of Yunnan Province, College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, No. 1076 Yuhua Road, Chenggong District, Kunming 650500, Yunnan, China.
  • 2 Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: qhwang668@sina.com.
  • 3 Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Harbin 150040, China.
  • 4 Center of Scientific experiment, Beijing University of Chinese Medicine, Beijing 10029, China.
  • 5 Guangdong Pharmaceutical University, Guangzhou 510006, China.
  • 6 Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Harbin 150040, China. Electronic address: hxkuang56@163.com.
Abstract

The leaves of Ilex Kudingcha, locally named "Kudingcha" in China, has been traditionally applied for treating obesity. Studies have demonstrated that the ethanol extract of Ilex kudingcha have anti-adipogenic effects. However, the constituent which was responsible for its anti-obesity and its underlying molecular mechanism has not yet been elucidated. This research explored the anti-obesity effect of kudinoside-D which was a main natural component of triterpenoid saponin from the ethanol extract of Ilex kudingcha, on lipid accumulation and the potential mechanism of action of adipogenesis in 3T3-L1 adipocytes. The adipocytes were treated with various concentrations of kudinoside D (0 to 40μM) during differentiation. The image-based Oil Red O staining analyses revealed that KD-D, dose dependently reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes with the IC50 is 59.49μM. Meanwhile, major adipogenic transcription factor Peroxisome Proliferator-activated Receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα) and sterol regulatory element-binding protein 1c (SREBP-1c) were significantly repressed as well as their target genes. The phosphorylation of AMP-activated protein kinase (AMPK) and its downstream target phosphorylated-acetyl CoA carboxylase (ACC) expression were also increased. In addition, the inhibitory effects of KD-D on the expressions of PPARγ and C/EBPα were weakened when cells were cotreated with AMPK Inhibitor Compound C. These results indicated KD-D exerts anti-adipogenic effects through modulation of adipogenic transcription factors via AMPK signaling pathway. And the current findings demonstrated that KD-D was a potential therapeutic candidate for alleviating obesity and hyperlipidemia.

Keywords

3T3-L1 preadipocytes; AMP-activated protein kinase (AMPK); Anti-adipogenic activity; Anti-obesity; Ilex Kudingcha; Kudinoside-D.

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