2. Academic Validation
  3. SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53

SRT2104 attenuates diabetes-induced aortic endothelial dysfunction via inhibition of P53

  • J Endocrinol. 2018 Apr;237(1):1-14. doi: 10.1530/JOE-17-0672.
Hao Wu 1 2,  Junduo Wu 3,  Shengzhu Zhou 4,  Wenlin Huang 5,  Ying Li 6,  Huan Zhang 7,  Junnan Wang 8,  Ye Jia 9
Affiliations

Affiliations

  • 1 Department of NephrologyThe Second Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
  • 2 The '973' National Basic Research Program of ChinaChangchun University of Chinese Medicine, Changchun, Jilin, People's Republic of China.
  • 3 Department of CardiologyThe Second Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
  • 4 Department of AnesthesiologyThe Second Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
  • 5 School of Science and TechnologyGeorgia Gwinnett College, Lawrenceville, Georgia, USA.
  • 6 Department of DermatologyAffiliated Hospital of Beihua University, Jilin, Jilin, People's Republic of China.
  • 7 Operating TheatreChina-Japan Union Hospital of Jilin University, Changchun, Jilin, People's Republic of China.
  • 8 Department of CardiologyThe Second Hospital of Jilin University, Changchun, Jilin, People's Republic of China wangjunnan@jlu.edu.cn jye@jlu.edu.cn.
  • 9 Department of NephrologyThe First Hospital of Jilin University, Changchun, Jilin, People's Republic of China wangjunnan@jlu.edu.cn jye@jlu.edu.cn.
PMID: 29371235 DOI: 10.1530/JOE-17-0672
Abstract

Endothelial dysfunction contributes to diabetic macrovascular complications. Sirtuin 1 (SIRT1) protects against diabetic vasculopathy. SRT2104 is a novel SIRT1 Activator and was not previously studied for its effects on diabetes-induced aortic endothelial dysfunction. Additionally, whether or to what extent deacetylation of P53, a substrate of SIRT1, is required for the effects of SIRT1 activation was unclear, given the fact that SIRT1 has multiple targets. Moreover, little was known about the pathogenic role of P53 in diabetes-induced aortic injury. To these ends, diabetes was induced by streptozotocin in C57BL/6 mice. The diabetic mice developed enhanced aortic contractility, oxidative stress, inflammation, P53 hyperacetylation and a remarkable decrease in SIRT1 protein, the effects of which were rescued by SRT2104. In HG-treated endothelial cells (ECs), P53 siRNA and SRT2104 produced similar effects on the induction of SIRT1 and the inhibition of P53 acetylation, oxidative stress and inflammation. Interestingly, SRT2104 failed to further enhance these effects in the presence of P53 siRNA. Moreover, P53 activation by nutlin3a completely abolished SRT2104's protection against HG-induced oxidative stress and inflammation. Further, forced activation of P53 by nutlin3a increased aortic contractility in the healthy mice and generated endothelial oxidative stress and inflammation in both the normal glucose-cultured ECs and the aortas of the healthy mice. Collectively, the present study demonstrates that P53 deacetylation predominantly mediates SRT2104's protection against diabetes-induced aortic endothelial dysfunction and highlights the pathogenic role of P53 in aortic endothelial dysfunction.

Keywords

P53; SIRT1; aorta; diabetes; endothelium.

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