2. Academic Validation
  3. The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR

The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR

  • Sci Rep. 2018 Feb 12;8(1):2848. doi: 10.1038/s41598-018-21116-3.
Jin Liu 1 2 3,  Xiaodan Zhao 1 2,  Dandan Pei 1 2 4,  Guo Sun 1 2,  Ye Li 1 2 3,  Chunhui Zhu 1 2 3,  Cui Qiang 1 2,  Junyi Sun 1 2 3,  Jianfeng Shi 1 2 5,  Yan Dong 6,  Jianzhong Gou 1 2 3,  Sicen Wang 7,  Ang Li 8 9 10 11
Affiliations

Affiliations

  • 1 Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 2 Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 3 Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 4 Department of Prothodontics, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 5 Research Center of Stomatology, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 6 The Second Affiliated Hospital, Xi'an Jiaotong University, 157 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China.
  • 7 School of Pharmacy, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710 061, Shannxi, People's Republic of China. wangsc@mail.xjtu.edu.cn.
  • 8 Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China. drliang@mail.xjtu.edu.cn.
  • 9 Clinical Research Center of Shannxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China. drliang@mail.xjtu.edu.cn.
  • 10 Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China. drliang@mail.xjtu.edu.cn.
  • 11 Research Center of Stomatology, College of Stomatology, Xi'an Jiaotong University, 98 XiWu Road, Xi'an, Shannxi, 710004, People's Republic of China. drliang@mail.xjtu.edu.cn.
PMID: 29434321 DOI: 10.1038/s41598-018-21116-3
Abstract

Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.

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