2. Academic Validation
  3. Phospholipid-flipping activity of P4-ATPase drives membrane curvature

Phospholipid-flipping activity of P4-ATPase drives membrane curvature

  • EMBO J. 2018 May 2;37(9):e97705. doi: 10.15252/embj.201797705.
Naoto Takada 1,  Tomoki Naito 1,  Takanari Inoue 2,  Kazuhisa Nakayama 1,  Hiroyuki Takatsu 1,  Hye-Won Shin 3
Affiliations

Affiliations

  • 1 Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
  • 2 Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
  • 3 Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan shin@pharm.kyoto-u.ac.jp.
PMID: 29599178 DOI: 10.15252/embj.201797705
Abstract

P4-ATPases are phospholipid flippases that translocate Phospholipids from the exoplasmic/luminal to the cytoplasmic leaflet of biological membranes. All P4-ATPases in yeast and some in Other organisms are required for membrane trafficking; therefore, changes in the transbilayer lipid composition induced by flippases are thought to be crucial for membrane deformation. However, it is poorly understood whether the phospholipid-flipping activity of P4-ATPases can promote membrane deformation. In this study, we assessed membrane deformation induced by flippase activity via monitoring the extent of membrane tubulation using a system that allows inducible recruitment of Bin/amphiphysin/Rvs (BAR) domains to the plasma membrane (PM). Enhanced phosphatidylcholine-flippase activity at the PM due to expression of ATP10A, a member of the P4-ATPase family, promoted membrane tubulation upon recruitment of BAR domains to the PM This is the important evidence that changes in the transbilayer lipid composition induced by P4-ATPases can deform biological membranes.

Keywords

BAR domain; curvature; flippase; lipid; plasma membrane.

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