2. Academic Validation
  3. Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models

Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models

  • Methods Mol Biol. 2018:1790:209-218. doi: 10.1007/978-1-4939-7860-1_16.
Jessica Swanner 1,  Ravi Singh 2 3
Affiliations

Affiliations

  • 1 Department of Cancer Biology, Wake Forest School of Medicine, Winston Salem, NC, USA.
  • 2 Department of Cancer Biology, Wake Forest School of Medicine, Winston Salem, NC, USA. rasingh@wakehealth.edu.
  • 3 Comprehensive Cancer Center of Wake Forest Baptist Medical Center, Winston Salem, NC, USA. rasingh@wakehealth.edu.
PMID: 29858794 DOI: 10.1007/978-1-4939-7860-1_16
Abstract

Silver nanoparticles (AgNPs) have a high affinity for sulfhydryl (thiol) groups, which can be exploited for functionalization with various tracking and targeting moieties. Here, we describe how to reliably and reproducibly functionalize AgNPs with the fluorescent moiety cyanine3-polyethelyne glycol (5000 molecular weight)-thiol (Cy3-PEG5000-SH). We also demonstrate how to purify and characterize Cy3-functionalized AgNPs (Cy3-AgNPs). Additionally, we describe how these Cy3-AgNPs can be imaged in 2D and 3D tumor models, providing insight into cellular localization and diffusion through a tumor spheroid, respectively.

Keywords

3D tumor models; Fluorescence; Imaging; Nanomaterials; Silver.

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