2. Academic Validation
  3. Identification of Rubisco anxiolytic-like peptides (rALPs) by comprehensive analysis of spinach green leaf protein digest

Identification of Rubisco anxiolytic-like peptides (rALPs) by comprehensive analysis of spinach green leaf protein digest

  • Biochem Biophys Res Commun. 2018 Nov 10;505(4):1050-1056. doi: 10.1016/j.bbrc.2018.09.195.
Saeko Kimura 1,  Tomoki Uchida 1,  Yuki Tokuyama 1,  Mayumi Hosokawa 1,  Junya Nakato 1,  Atsushi Kurabayashi 2,  Masaru Sato 2,  Hideyuki Suzuki 2,  Ryuhei Kanamoto 1,  Kousaku Ohinata 3
Affiliations

Affiliations

  • 1 Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan.
  • 2 Department of Research & Development, Kazusa DNA Research Institutes, Japan.
  • 3 Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan. Electronic address: ohinata@kais.kyoto-u.ac.jp.
PMID: 30314694 DOI: 10.1016/j.bbrc.2018.09.195
Abstract

Rubisco, an Enzyme for photosynthetic carbon dioxide fixation, is a major green leaf protein and known as the most abundant protein on the Earth. We found that Rubisco digested mimicking gastrointestinal enzymatic conditions exhibited anxiolytic-like effects after oral administration in mice. Based on a comprehensive peptide analysis of the digest using nanoLC-Orbitrap-MS and the structure-activity relationship of known anxiolytic-like Peptides, we identified SYLPPLTT, SYLPPLT and YHIEPV [termed Rubisco anxiolytic-like peptide (rALP)-1, rALP-1(1-7) and rALP-2, respectively], which exhibited potent anxiolytic-like effects after oral administration. The anxiolytic-like effects of rALP-1/rALP-1(1-7) were blocked by a serotonin 5-HT1A receptor antagonist, whereas rALP-2-induced effects were inhibited by a δ-opioid receptor antagonist. In conclusion, novel Rubisco-derived anxiolytic-like Peptides, rALP-1/rALP-1(1-7) and rALP-2, act via independent neural pathways.

Keywords

Anxiolytic-like effect; Comprehensive analysis; Green leaf; Oral administration; Peptide; Rubisco.

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