1. Academic Validation
  2. Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol

  • Braz J Microbiol. 2019 Jan;50(1):79-84. doi: 10.1007/s42770-019-00040-2.
Xiuwen Wang 1 Xiaohua Zhang 1 Qingshou Yao 1 Dongliang Hua 2 Jiayang Qin 3
Affiliations

Affiliations

  • 1 College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China.
  • 2 Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan, China.
  • 3 College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China. qinjysdu@163.com.
Abstract

Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 upregulated protein spots with a > 2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The upregulated proteins under sclareol stress are involved in carbon metabolism and nitrogen metabolism, and replication, transcription, and translation processes. Eighteen upregulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.

Keywords

Ambroxide; Comparative proteomic; Hyphozyma roseonigra; Sclareol; Sclareol glycol.

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