1. Academic Validation
  2. Evaluation of Resmethrin Toxicity to Neonatal Testes in Organ Culture

Evaluation of Resmethrin Toxicity to Neonatal Testes in Organ Culture

  • Toxicol Sci. 2020 Jan 1;173(1):53-64. doi: 10.1093/toxsci/kfz212.
Hyun-Jung Park 1 Won-Young Lee 2 Mingtian Zhang 1 Kwon-Ho Hong 1 Chankyu Park 1 Jin-Hoi Kim 1 Hyuk Song 1
Affiliations

Affiliations

  • 1 Department of Stem Cell and Regenerative Biology, Konkuk University, Gwangjin-gu, Seoul 05029, Republic of Korea.
  • 2 Department of Beef Science, Korea National College of Agricultures and Fisheries, Jeonju-si, Jeonbuk 54874, Republic of Korea.
Abstract

Resmethrin is a widely used pyrethroid insecticide, which causes low toxicity in mammals. However, its toxicity in testes has not been fully investigated. Therefore, we evaluated the toxicity of resmethrin in mouse testes using an in vitro organ culture. Mouse testicular fragments (MTFs) derived from neonates were cultured in medium containing resmethrin for 30 days. Effects on spermatogenesis in the cultured testes were investigated as functions of both time and dose. Resmethrin significantly downregulated the transcription levels of marker genes for spermatogonia and the number of spermatogenic germ cells relative to those of the controls, according to quantitative PCR and immunostaining. In addition, spermatocyte was observed in the control, but not in 50 μM resmethrin-exposed cultures. Levels of the SYCP3 meiotic marker and phosphorylated H2AX decreased by resmethrin treatment, as observed by Western blotting. Toxic or apoptotic effects of resmethrin in Sertoli and Leydig cells from MTFs were not observed by immunostaining and Tunnel assay. No changes in the expression of steroidogenic Enzymes were noted. Apoptosis was only detected in the germ cells of resmethrin-treated MTFs. Thus, the highest dose of resmethrin tested (50 μM) completely inhibited spermatogenesis, because of Apoptosis of germ cells and spermatocytes. Although the in vivo toxicity of resmethrin has not yet been studied in detail, significant evidence for cytotoxicity was observed in our organ cultures. This methodological approach is useful for the study of reproductive toxicity before proceeding to animal models, as it greatly reduces the use of laboratory Animals.

Keywords

mouse testicular fragments; organ culture; resmethrin; toxicity.

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