2. Academic Validation
  3. Toxic effects of the Emamectin Benzoate exposure on cultured human bronchial epithelial (16HBE) cells

Toxic effects of the Emamectin Benzoate exposure on cultured human bronchial epithelial (16HBE) cells

  • Environ Pollut. 2020 Feb;257:113618. doi: 10.1016/j.envpol.2019.113618.
Chenguang Niu 1,  Chunli Wang 2,  Guangyao Wu 3,  Jingnan Yang 3,  Yanan Wen 4,  Shuangshuang Meng 4,  Xuhong Lin 4,  Xiaobin Pang 5,  Lei An 6
Affiliations

Affiliations

  • 1 Key Laboratory of Clinical Resources Translation, The First Affiliated Hospital of Henan University, Kaifeng, 475000, Henan, China.
  • 2 Pharmaceutical Institute, School of Pharmacy, Henan University, Kaifeng, 475000, Henan, China.
  • 3 Translational Medicine Center, Huaihe Hospital of Henan University, Kaifeng, 475000, Henan, China.
  • 4 Department of Clinical Laboratory, Huaihe Hospital of Henan University, Kaifeng, 475000, Henan, China.
  • 5 Pharmaceutical Institute, School of Pharmacy, Henan University, Kaifeng, 475000, Henan, China. Electronic address: pxb@vip.henu.edu.cn.
  • 6 Translational Medicine Center, Huaihe Hospital of Henan University, Kaifeng, 475000, Henan, China; Institutes of Biomedical Sciences, Henan Medical School, Henan University, Kaifeng, 475000, Henan, China. Electronic address: hndxal@163.com.
PMID: 31784274 DOI: 10.1016/j.envpol.2019.113618
Abstract

Pesticides pollution has caused serious environmental problems in recent years, and mounting evidence has shown that more and more insecticides have serious risk in human health. Emamectin Benzoate formally regarded as a highly safety insecticide based on its exclusive targets, but the cytotoxicity to human lung was ignored for a long time. In the present study, bioassay experiments were used to assess the toxicity of the Emamectin Benzoatein on human non-target cells including cell viability assay, DNA damage assay, flow cytometer assay and western blotting assay. The results indicated that Emamectin Benzoatecan cause the inhibition of the proliferation, cytochrome c release, activation of Caspase-3/9 and increase Bax/Bcl-2 ratio, which means it induced the cytotoxicity on 16HBE cells associated with the mitochondrial Apoptosis. Besides, the DNA damge caused by the Emamectin Benzoate suggest it has a potential genotoxic effect on human lung cells.

Keywords

Cytotoxicity; Emamectin benzoate; Human bronchial epithelial (16HBE) cells; Mitochondrial apoptosis; Pesticides exposure.

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