Emamectin Benzoate (Synonyms: MK-244)

Cat. No.: HY-B0837 Purity: 99.71%: FAQs
Data Sheet SDS COA Handling Instructions

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Emamectin Benzoate (MK-244) is an orally active nervoussystem toxicant by binding g-aminobutyric (GABA) receptor in insects. Emamectin Benzoate is one of semi-synthetic derivative of Avermectin (HY-15311) with a broadspectrum of insecticidal and acaricidal activity. Emamectin Benzoate induces ROS-mediated DNA damage and cell apoptosis. Emamectin Benzoate, a mixture of the natural Emamectin B1a benzoate and Emamectin B1b benzoate, has the main component of Emamectin B1a benzoate.

For research use only. We do not sell to patients.

Emamectin Benzoate Chemical Structure

CAS No. : 155569-91-8

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 44	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Mite

In Vitro

Emamectin Benzoate (MK-244; 2.5-40 μM; 12 and 24 h) decreases cell viability in a time- and dose-dependent manner^[1].
Emamectin Benzoate (2.5-20 μM; 24 hours) induces apoptosis and DNA damage in 16HBE cells. Emamectin Benzoate induces ROS generation in 16HBE cells^[1].
Emamectin Benzoate (2.5-20 μM; 12 hours) increases the amounts of cytochrome-c, caspase-3, cas-pase-9, cleaved-PARP, Bax/Bcl-2^[1].
Emamectin Benzoate (2.5, 5, 10, 15 μM; 72 h) inhibits cell viability with an IC₅₀ of 3.72 μM in Trichoplusia Tn5B1-4 cell. Emamectin Benzoat induces chromatin condensation in nuclei and cell apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	human normal bronchial epithelial cell line 16HBE
Concentration:	2.5, 5, 7.5,10,15, 20, 40 μM
Incubation Time:	12 and 24 hours
Result:	Decreased cell viability in a time- and dose-dependent manner wirh IC₅₀s of 11.88 μM and 9.67 μM in 12 and 24 hours, respectively.

Apoptosis Analysis^[1]

Cell Line:	human normal bronchial epithelial cell line 16HBE
Concentration:	2.5, 5, 10, 20 μM
Incubation Time:	24 hours
Result:	Induced apoptosis and caused chromatin shrinkage and nuclear fragmentation.

Western Blot Analysis^[1]

Cell Line:	human normal bronchial epithelial cell line 16HBE
Concentration:	2.5, 5, 10, 20 μM
Incubation Time:	12 hours
Result:	Increased the amounts of cytochrome-c, caspase-3, cas-pase-9, cleaved-PARP, Bax/Bcl-2.

In Vivo

Emamectin Benzoate (MK-244; oral; 25-100 mg/kg/day; for 14 days) causes a marked induction of oxidative damage in liver tissue^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10 weeks old Swiss albino male mice (25-30 g)^[3]
Dosage:	25, 50, 100 mg/kg
Administration:	Oral; daily; for 14 days
Result:	Caused a marked induction of oxidative damage in liver tissue as demonstrated by an increased level of TBARS and reduced GSH level.

Molecular Weight

1008.24(Based on Emamectin B1a Benzoate)

Formula

C₄₉H₇₅NO₁₃.C₇H₆O₂

CAS No.

155569-91-8

Appearance

Solid

Color

White to off-white

SMILES

C[C@H]1O[C@@H](O[C@@]2([H])[C@H](C)O[C@@H](O[C@]([C@@H](C)/C=C/C=C3CO[C@@]4([H])[C@]\3(O)[C@H]5C=C(C)[C@H]4O)([H])/C(C)=C/C[C@@H]6C[C@H](OC5=O)C[C@]7(C=C[C@H](C)[C@@H](C(C)C)O7)O6)C[C@@H]2OC)C[C@@H](OC)[C@@H]1NC.C[C@H]8O[C@@H](O[C@@]9([H])[C@H](C)O[C@@H](O[C@]([C@@H](C)/C=C/C=C%10CO[C@@]%11([H])[C@]\%10(O)[C@H]%12C=C(C)[C@H]%11O)([H])/C(C)=C/C[C@@H]%13C[C@H](OC%12=O)C[C@]%14(C=C[C@H](C)[C@@H]([C@H](CC)C)O%14)O%13)C[C@@H]9OC)C[C@@H](OC)[C@@H]8NC.O=C(O)C%15=CC=CC=C%15

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.71%

Select Batch:

Data Sheet (287 KB) SDS (623 KB)

COA (268 KB) HNMR (397 KB) RP-HPLC (247 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Chenguang Niu, et al. Toxic effects of the Emamectin Benzoate exposure on cultured human bronchial epithelial (16HBE) cells. Environ Pollut. 2020 Feb;257:113618. [Content Brief]

[2]. Shaorong Luan, et al. Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells. Chem Biol Interact. 2017 Aug 1;273:90-98. [Content Brief]

[3]. Özge Temiz, et al. Biopesticide emamectin benzoate in the liver of male mice: evaluation of oxidative toxicity with stress protein, DNA oxidation, and apoptosis biomarkers. Environ Sci Pollut Res Int. 2020 Jun;27(18):23199-23205. [Content Brief]

Emamectin Benzoate Related Classifications

Neurological Disease Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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