2. Academic Validation
  3. WNK4-SPAK modulates lipopolysaccharide-induced macrophage activation

WNK4-SPAK modulates lipopolysaccharide-induced macrophage activation

  • Biochem Pharmacol. 2020 Jan:171:113738. doi: 10.1016/j.bcp.2019.113738.
Chin-Mao Hung 1,  Chung-Kan Peng 2,  Sung-Sen Yang 3,  Hao-Ai Shui 4,  Kun-Lun Huang 5
Affiliations

Affiliations

  • 1 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan; Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan.
  • 2 Division of Pulmonary and Critical Care, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • 3 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan; Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • 4 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
  • 5 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan; Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical Center, Taipei, Taiwan; Division of Pulmonary and Critical Care, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. Electronic address: kun@mail.ndmctsgh.edu.tw.
PMID: 31786261 DOI: 10.1016/j.bcp.2019.113738
Abstract

Dysregulation of alveolar macrophage activation has been recognized as the major mechanism in the pathogenesis of acute lung injury. The aim of the present study was to investigate the role of NKCC1 regulating mechanism in modulating macrophage activation. Knockout (SPAK-/- and WNK4-/-) and knockin (WNK4D561A/+) mice were used in this study. LPS induced expression of p-NKCC1 and activation of NFκB in the primary culture of alveolar macrophages. WNK4 or SPAK knockout suppressed p-NKCC1 expression and inflammation cascade activation, whereas WNK4 knockin enhanced these responses. Intrapulmonary administration of LPS induced in vivo expression and phosphorylation of NKCC1 in alveolar inflammation cells and caused a shift in the cell population from macrophage to neutrophil predominance. WNK4 or SPAK knockout attenuated the LPS-induced alveolar cell-population shifting, macrophage NKCC1 phosphorylation, and acute lung injury, whereas WNK4 knockin augmented the inflammatory response. In summary, our results demonstrated the presence of NKCC1 in alveolar macrophage, which is inducible by lipopolysaccharide. Our results also showed showed that the WNK4-SPAK-NKCC1 cascade plays an important role in modulating macrophage activation to regulate LPS-induced lung inflammation and lung injury.

Keywords

Alveolar macrophage; Inflammation; Na-K-Cl cotransporter-1; SPAK; WNK4.

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