2. Academic Validation
  3. Chronic exposure of zebrafish (Danio rerio) to flutolanil leads to endocrine disruption and reproductive disorders

Chronic exposure of zebrafish (Danio rerio) to flutolanil leads to endocrine disruption and reproductive disorders

  • Environ Res. 2020 May:184:109310. doi: 10.1016/j.envres.2020.109310.
Miaomiao Teng 1,  Chen Wang 2,  Min Song 3,  Xiangguang Chen 1,  Jie Zhang 1,  Chengju Wang 4
Affiliations

Affiliations

  • 1 College of Sciences, China Agricultural University, Beijing, 100193, China.
  • 2 State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
  • 3 Tai'an Academy of Agricultural Sciences, Taian, 271000, Shandong Province, China.
  • 4 College of Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: wangchengju@cau.edu.cn.
PMID: 32163770 DOI: 10.1016/j.envres.2020.109310
Abstract

The pesticide flutolanil has been detected in fish and aquatic environments, but its potential impact on the endocrine function is unknown. In this study, two-month zebrafish were exposed to the environmentally relevant concentrations of flutolanil for 60 days to examine the reproductive endpoints on the gonad endocrine system. Increased 17 beta-estradiol (17β-E2) content and 17β-E2/T ratio and decreased testosterone (T) in male suggested that flutolanil produces the estrogenic effect. In support of this view, vitellogene (vtg1, vtg2) and Cytochrome P450 aromatase 19a (cyp19a) expression were up-regulated in the male liver. The levels of global DNA methylation were increased in ovary. Parental zebrafish exposure to different concentrations of flutolanil affected the offspring development as shown by short body length, and increased mortality. Thus, these results demonstrate that flutolanil exposure results in gonad endocrine disruption, decreased reproduction, and developmental toxicity in F1, highlighting the importance of assessing the potential environmental risks of flutolanil application.

Keywords

DNA methylation; Endocrine disruption; Flutolanil; Reproduction; Zebrafish.

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