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  2. Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential

  • Cells. 2020 Apr 22;9(4):1045. doi: 10.3390/cells9041045.
Kai Qiu 1 2 Doudou Xu 1 Liqi Wang 1 Xin Zhang 1 2 Ning Jiao 1 2 Lu Gong 1 Jingdong Yin 1
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China.
  • 2 College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Abstract

This study is aimed at exploring the mechanism underlying the homeostasis between myogenesis and adipogenesis in skeletal muscle using a special porcine model with a distinct phenotype on muscle growth rate and intramuscular fat deposition. Differentiation potential of muscle-derived Myo-lineage cells of lean-type pigs was significantly enhanced relative to obese-type pigs, while that of their Adi-lineage cells was similar. Single-cell RNA Sequencing revealed that lean-type pigs reserved a higher proportion of Myo-lineage cells in skeletal muscle relative to obese-type pigs. Besides, Myo-lineage cells of the lean-type pig settled closer to the original stage of muscle-derived progenitor cells. Proteomics analysis found that differentially expressed proteins between two sources of Myo-lineage cells are mainly involved in muscle development, cell proliferation and differentiation, ion homeostasis, Apoptosis, and the MAPK signaling pathway. The regulation of intracellular ion homeostasis, CA2+ in particular, significantly differed between two sources of Myo-lineage cells. CA2+ concentration in both cytoplasm and endoplasmic reticulum was lower in Myo-lineage cells of lean-type pigs relative to obese-type pigs. In conclusion, a higher proportion and stronger differentiation capacity of Myo-lineage cells are the main causes for the higher capability of myogenic differentiation and lower intramuscular fat deposition. Relative low concentration of cellular CA2+ is advantageous for Myo-lineage cells to keep a potent differentiation potential.

Keywords

Ca2+; differentiation potential; ion homeostasis; muscle health; myogenesis.

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