2. Academic Validation
  3. Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography

Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography

  • ACS Appl Mater Interfaces. 2020 Jul 1;12(26):29000-29012. doi: 10.1021/acsami.0c05432.
T Sansen 1,  D Sanchez-Fuentes 2,  R Rathar 1 2,  A Colom-Diego 3,  F El Alaoui 1,  J Viaud 4,  M Macchione 5,  S de Rossi 6,  S Matile 5,  R Gaudin 1,  V Bäcker 6,  A Carretero-Genevrier 2,  L Picas 1
Affiliations

Affiliations

  • 1 Institut de Recherche en Infectiologie de Montpellier (IRIM), CNRS UMR 9004-Université de Montpellier, 34293 Montpellier, France.
  • 2 Institut d'Électronique et des Systèmes (IES), CNRS UMR 5214-Université de Montpellier, 34097 Montpellier, France.
  • 3 Biochemistry Department and School of Chemistry and Biochemistry and Swiss National Centre for Competence in Research in Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland.
  • 4 Institute of Cardiovascular and Metabolic Diseases (I2MC-UMR1048), Inserm and Université Toulouse 3, Avenue Jean Poulhès BP84225, 31432 Cedex 04 Toulouse, France.
  • 5 School of Chemistry and Biochemistry and Swiss National Centre for Competence in Research in Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland.
  • 6 MRI Imaging Facility, UMS BioCampus Montpellier, 34000 Montpellier, France.
PMID: 32464046 DOI: 10.1021/acsami.0c05432
Abstract

Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and benchtop method allowing one to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.

Keywords

biointerfaces; cellular membranes; microscopy; nanostructured materials; proteins.

Figures
Products