2. Academic Validation
  3. Isolation and biological activity of agrostophillinol from kaffir lime (Citrus hystrix) leaves

Isolation and biological activity of agrostophillinol from kaffir lime (Citrus hystrix) leaves

  • Bioorg Med Chem Lett. 2020 Jul 15;30(14):127256. doi: 10.1016/j.bmcl.2020.127256.
Songyot Anuchapreeda 1,  Riki Anzawa 2,  Natsima Viriyaadhammaa 3,  Waranya Neimkhum 4,  Wantida Chaiyana 5,  Siriporn Okonogi 6,  Toyonobu Usuki 7
Affiliations

Affiliations

  • 1 Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan; Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: sanuchapreeda@gmail.com.
  • 2 Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan.
  • 3 Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  • 4 Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutprakarn 10250, Thailand.
  • 5 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand.
  • 6 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: okng2000@gmail.com.
  • 7 Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan. Electronic address: t-usuki@sophia.ac.jp.
PMID: 32527555 DOI: 10.1016/j.bmcl.2020.127256
Abstract

The leaves of the kaffir lime (Citrus hystrix) are commonly used in cuisine and folk medicine. The aim of this study was to isolate a bioactive compound in kaffir lime leaves and characterize its biological activity. The compound was isolated from a hexane fractional extract and identified as agrostophillinol. This is the first report of agrostophillinol isolated from kaffir lime leaves. In terms of cytotoxicity, agrostophillinol exhibited IC50 values of 36.27 ± 7.30 and 53.44 ± 10.63 μg/mL against EoL-1 and HL60 cells, respectively. Agrostophillinol also exhibited potent anti-inflammatory activity, significantly inhibiting IL-6 secretion.

Keywords

Agrostophillinol; Anti-inflammatory; Anticancer; Antioxidant; Citrus hystrix; Kaffir lime.

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