1. Academic Validation
  2. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf

Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf

  • J Nat Med. 2020 Sep;74(4):732-740. doi: 10.1007/s11418-020-01427-4.
Li-Hua Mu  # 1 Li-Hua Wang  # 1 Yu-Ning Wang 2 Ping Liu 3 Can Yan 4 5
Affiliations

Affiliations

  • 1 Medical Supplies Center, Chinese PLA General Hospital, Beijing, 100853, China.
  • 2 First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
  • 3 Medical Supplies Center, Chinese PLA General Hospital, Beijing, 100853, China. cpi301@163.com.
  • 4 Department of Basic Theory of Chinese Medicine, School of Pre-Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510060, China. gzyancan@hotmail.com.
  • 5 The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510060, China. gzyancan@hotmail.com.
  • # Contributed equally.
Abstract

AG36 is a triterpenoid saponin from Ardisia gigantifolia stapf. Our recent studies proved that AG36 displayed prominent cytotoxicity against breast Cancer cells both in vitro and in vivo. However, whether AG36 has antiangiogenic properties is unknown. Therefore, in the present study, we evaluated the antiangiogenic effect of AG36 and the underlying mechanism. The results indicated that AG36 could significantly inhibit the proliferation, migration and invasion of human umbilical vein endothelial cells (HUVEC). Further antiangiogenic molecular mechanism investigation showed that AG36 significantly suppressed phosphorylated FAK and Akt, and downregulated the expressions of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR2/KDR/Flk-1) in HUVECs. PI3K Inhibitor (LY294002) and FAK Inhibitor (PF562271) pretreatment could markedly enhance AG36-induced inhibition of HUVEC proliferation and p-FAK suppression, respectively. In addition, AG36 inhibited the tumor growth in xenograft model and expressions of p-VEGFR2 and p-Akt in vivo. Molecular docking simulation indicated that AG36 formed hydrogen bonds and hydrophobic interactions within the ATP binding pocket of VEGFR2/KDR/Flk-1 kinase domain. The present study firstly revealed the high antiangiogenic potency and related underlying molecular of AG36, demonstrating that AG36 maybe a potential antiangiogenic Cancer therapy agent or lead candidate.

Keywords

Antiangiogenic activity; Ardisia gigantifolia; Human umbilical vein endothelial cells; Molecular docking; Triterpenoid saponin.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-10459
    99.68%, FAK/Pyk2 Inhibitor