1. Academic Validation
  2. Down-regulation of LECT2 promotes osteogenic differentiation of MSCs via activating Wnt/β-catenin pathway

Down-regulation of LECT2 promotes osteogenic differentiation of MSCs via activating Wnt/β-catenin pathway

  • Biomed Pharmacother. 2020 Oct;130:110593. doi: 10.1016/j.biopha.2020.110593.
Zhonghua Xu 1 Jin He 1 Xindie Zhou 2 Yi Zhang 3 Yong Huang 3 Nanwei Xu 3 Haoyu Yang 4
Affiliations

Affiliations

  • 1 Department of Orthopedics, Jintan Hospital Affiliated to Jiangsu University, Changzhou, 213200, China.
  • 2 Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213003, China. Electronic address: xindiezhou@163.com.
  • 3 Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213003, China.
  • 4 Department of Orthopedics, Wuxi 9th People's Hospital Affiliated to Soochow University, Wuxi, 214000, China. Electronic address: haoyu_yang@126.com.
Abstract

Osteoporosis is a result of the imbalance between osteoblasts and osteoclasts quantities, which is closely correlated with osteogenic differentiation (OD). Leucocyte cell-derived chemotaxin 2 (LECT2) has been reported as a regulatory factor in some chronic diseases such as hepatitis through mediating downstream target gene β-catenin. Additionally, Wnt/β-catenin is also the crucial modulatory signal pathway in OD. Mesenchymal stem cells (MSC) is a kind of mesodermal stem cells; its differentiation direction is discovered affected by Wnt/β-catenin. However, the function of LECT2 in osteoporosis still remains exploration, which encourages us to lucubrate its functional effect in regulating the OD of MSCs. In this study, we found that LECT2 was expressed at low level in MSCs with osteogenic differentiation, and knockdown of LECT2 would activate Wnt/β-catenin pathway and therefore promoting OD in MSCs. It is the first time to report that LECT2 participates in regulating OD via mediating Wnt/β-catenin. Our discovery would affirmatively help provide a novel strategy for the diagnosis and therapy methods for osteoporosis.

Keywords

Mesenchymal stem cells; Osteogenic differentiation; Osteoporosis; Wnt/β-catenin.

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