2. Academic Validation
  3. Effect of lipid shell composition in DSPG-based microbubbles on blood flow imaging with ultrasonography

Effect of lipid shell composition in DSPG-based microbubbles on blood flow imaging with ultrasonography

  • Int J Pharm. 2020 Nov 30;590:119886. doi: 10.1016/j.ijpharm.2020.119886.
Tamotsu Maruyama 1,  Mutsumi Sugii 1,  Daiki Omata 1,  Johan Unga 1,  Tadamitsu Shima 1,  Lisa Munakata 1,  Saori Kageyama 1,  Fumiko Hagiwara 1,  Yuno Suzuki 1,  Kazuo Maruyama 2,  Ryo Suzuki 3
Affiliations

Affiliations

  • 1 Laboratory of Drug and Gene Delivery Research, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan.
  • 2 Laboratory of Theranostics, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan.
  • 3 Laboratory of Drug and Gene Delivery Research, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan. Electronic address: r-suzuki@pharm.teikyo-u.ac.jp.
PMID: 32998031 DOI: 10.1016/j.ijpharm.2020.119886
Abstract

Diagnostic ultrasound is non-invasive and provides real-time imaging. Microbubbles (MBs) are ultrasound contrast agents used to observe small blood flow, such as tumor tissue. However, MBs have short blood flow imaging time. This study developed lipid-based microbubbles (LMBs) with longer blood flow imaging time by focusing on their shell composition. Liposome research reported that addition 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) to the lipid composition enhances Liposome membrane stability. Therefore, we introduced DSPG at different ratios into the LMBs lipid shell. Results showed that the lipid shell composition of MBs affects stability in vivo. 60% DSPG-containing LMBs (DSPG60-LMBs) have sustained blood flow imaging time compared with LMBs, which have Other DSPG ratios, Sonazoid® and SonoVue®. DSPG60-LMBs also showed less uptake into the liver compared with Sonazoid®. Therefore, DSPG60-LMBs can have long blood flow imaging time and can be effective diagnostic agents in ultrasound imaging.

Keywords

Blood flow imaging; DSPG; Diagnostics; Microbubbles; Ultrasound contrast agent.

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