2. Academic Validation
  3. Mechanochemical feedback mediates tissue bending required for seedling emergence

Mechanochemical feedback mediates tissue bending required for seedling emergence

  • Curr Biol. 2021 Mar 22;31(6):1154-1164.e3. doi: 10.1016/j.cub.2020.12.016.
Kristoffer Jonsson 1,  Rahul S Lathe 2,  Daniel Kierzkowski 3,  Anne-Lise Routier-Kierzkowska 3,  Olivier Hamant 4,  Rishikesh P Bhalerao 5
Affiliations

Affiliations

  • 1 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90187 Umeå, Sweden. Electronic address: kristoffer.jonsson@slu.se.
  • 2 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90187 Umeå, Sweden.
  • 3 IRBV, Department of Biological Sciences, University of Montreal, 4101 Sherbrooke Est, Montréal H1X 2B2, QC, Canada.
  • 4 Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, Lyon, France.
  • 5 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 90187 Umeå, Sweden. Electronic address: rishi.bhalerao@slu.se.
PMID: 33417884 DOI: 10.1016/j.cub.2020.12.016
Abstract

Tissue bending is vital to plant development, as exemplified by apical hook formation during seedling emergence by bending of the hypocotyl. How tissue bending is coordinated during development remains poorly understood, especially in Plants where cells are attached via rigid cell walls. Asymmetric distribution of the plant hormone Auxin underlies differential cell elongation during apical hook formation. Yet the underlying mechanism remains unclear. Here, we demonstrate spatial correlation between asymmetric Auxin distribution, methylesterified homogalacturonan (HG) pectin, and mechanical properties of the epidermal layer of the hypocotyl in Arabidopsis. Genetic and cell biological approaches show that this mechanochemical asymmetry is essential for differential cell elongation. We show that asymmetric Auxin distribution underlies differential HG methylesterification, and conversely changes in HG methylesterification impact the Auxin response domain. Our results suggest that a positive feedback loop between Auxin distribution and HG methylesterification underpins asymmetric cell wall mechanochemical properties to promote tissue bending and seedling emergence.

Keywords

Arabidopsis; PIN proteins; PMEI; apical hook; auxin; cell wall; development; differential growth; pectin methylesterification.

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