2. Academic Validation
  3. Imidazopyridine-Based 5-HT6 Receptor Neutral Antagonists: Impact of N1-Benzyl and N1-Phenylsulfonyl Fragments on Different Receptor Conformational States

Imidazopyridine-Based 5-HT6 Receptor Neutral Antagonists: Impact of N1-Benzyl and N1-Phenylsulfonyl Fragments on Different Receptor Conformational States

  • J Med Chem. 2021 Jan 28;64(2):1180-1196. doi: 10.1021/acs.jmedchem.0c02009.
David Vanda 1,  Vittorio Canale 2,  Severine Chaumont-Dubel 3,  Rafał Kurczab 4,  Grzegorz Satała 4,  Paulina Koczurkiewicz-Adamczyk 2,  Martyna Krawczyk 4,  Wojciech Pietruś 4,  Klaudia Blicharz 2,  Elżbieta Pękala 2,  Andrzej J Bojarski 4,  Piotr Popik 4,  Philippe Marin 3,  Miroslav Soural 1 5,  Paweł Zajdel 6
Affiliations

Affiliations

  • 1 Faculty of Science, Department of Organic Chemistry, Palacký University, 17. listopadu 12, Olomouc 771 46, Czech Republic.
  • 2 Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, Kraków 30-688, Poland.
  • 3 Institut de Génomique Fonctionnelle, Univ. Montpellier, INSERM, CNRS, 141 Rue de la Cardonille, Montpellier 34-094, France.
  • 4 Maj Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna Street, Kraków 31-343, Poland.
  • 5 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, Olomouc 779 00, Czech Republic.
  • 6 Faculty of Pharmacy, Department of Organic Chemistry, Jagiellonian University Medical College, 9 Medyczna Street, Kraków 30-688, Poland.
PMID: 33439019 DOI: 10.1021/acs.jmedchem.0c02009
Abstract

G-protein coupled receptors (GPCRs) exist in an equilibrium of multiple conformational states, including different active states, which depend on the nature of the bound ligand. In consequence, different conformational states can initiate specific signal transduction pathways. The study identified compound 7e, which acts as a potent 5-hydroxytryptamine type 6 receptor (5-HT6R) neutral antagonist at Gs and does not impact neurite growth (process controlled by CDK5). MD simulations highlighted receptor conformational changes for 7e and inverse agonist PZ-1444. In cell-based assays, neutral antagonists of the 5-HT6R (7e and CPPQ), but not inverse agonists (SB-258585, intepirdine, PZ-1444), displayed glioprotective properties against 6-hydroxydopamine-induced and doxorubicin-induced cytotoxicity. These suggest that targeting the activated conformational state of the 5-HT6R with neutral antagonists implicates the protecting properties of astrocytes. Additionally, 7e prevented scopolamine-induced learning deficits in the novel object recognition test in rats. We propose 7e as a probe for further understanding of the functional outcomes of different states of the 5-HT6R.

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