2. Academic Validation
  3. UDP-Glucose Dehydrogenases: Identification, Expression, and Function Analyses in Upland Cotton ( Gossypium hirsutum)

UDP-Glucose Dehydrogenases: Identification, Expression, and Function Analyses in Upland Cotton ( Gossypium hirsutum)

  • Front Genet. 2021 Jan 11;11:597890. doi: 10.3389/fgene.2020.597890.
Tingting Jia 1,  Qun Ge 1,  Shuya Zhang 1,  Zhen Zhang 1,  Aiying Liu 1,  Senmiao Fan 1,  Xiao Jiang 1,  Yulong Feng 2,  Lipeng Zhang 1,  Doudou Niu 1,  Shen Huang 3,  Wankui Gong 1,  Youlu Yuan 1 2,  Haihong Shang 1 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
  • 2 Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China.
  • 3 Zhengzhou University of Light Industry College of Food and Bioengineering, Zhengzhou, China.
PMID: 33505427 DOI: 10.3389/fgene.2020.597890
Abstract

UDP-glucose dehydrogenase (UGD; EC1.1.1.22) is a NAD+-dependent Enzyme that catalyzes the two-fold oxidation of UDP-glucose (UDP-Glc) to produce UDP-glucuronic acid and plays an important role in plant cell wall synthesis. A total of 42 UGD genes from four Gossypium genomes including G. hirsutum, G. arboretum, G. barbadense, and G. raimondii were identified and found that the UGD gene family has conservative evolution patterns in gene structure and protein domain. The growth of fibers can be effectively promoted after adding the UDP-Glc to the medium, and the GhUGD gene expression enhanced. In addition, the transgenic Arabidopsis lines over-expressing GH_D12G1806 had longer root lengths and higher gene expression level than the wild-type Plants of Columbia-0. These results indicated that UGD may play important roles in cotton fiber development and has a guiding significance for dissecting fiber development mechanism.

Keywords

UDP-glucose dehydrogenase; UDP-glucuronic acid; cotton fiber; fiber development; transgenic Arabidopsis.

Figures
Products