2. Academic Validation
  3. The effects of different potato dextrose agar media on secondary metabolite production in Fusarium

The effects of different potato dextrose agar media on secondary metabolite production in Fusarium

  • Int J Food Microbiol. 2021 Jun 2:347:109171. doi: 10.1016/j.ijfoodmicro.2021.109171.
Klaus Ringsborg Westphal 1,  Søren Heidelbach 1,  Emil Juel Zeuner 1,  Marie Riisgaard-Jensen 1,  Morten Eneberg Nielsen 1,  Sofie Zacho Vestergaard 1,  Nicolai Sundgaard Bekker 1,  Jesper Skovmark 1,  Christian Kjær Olesen 1,  Katrine Hartmann Thomsen 1,  Sara Kramer Niebling 1,  Jens Laurids Sørensen 2,  Teis Esben Sondergaard 3
Affiliations

Affiliations

  • 1 Aalborg University, Department of Chemistry and Bioscience, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
  • 2 Aalborg University, Department of Chemistry and Bioscience, Niels Bohrsvej 8, 6700 Esbjerg, Denmark.
  • 3 Aalborg University, Department of Chemistry and Bioscience, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark. Electronic address: tes@bio.aau.dk.
PMID: 33872940 DOI: 10.1016/j.ijfoodmicro.2021.109171
Abstract

Potatoes contain several nutrients essential for Fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites. This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum). Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.

Keywords

Fusarium; Mycotoxins; Potato dextrose agar; Regulation; Secondary metabolites; Solanine.

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