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  2. Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish

Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish

  • Food Chem. 2021 Oct 30;360:129814. doi: 10.1016/j.foodchem.2021.129814.
Dian-Guang Zhang 1 Tao Zhao 1 Christer Hogstrand 2 Han-Mei Ye 1 Xiao-Jian Xu 1 Zhi Luo 3
Affiliations

Affiliations

  • 1 Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, China.
  • 2 Diabetes and Nutritional Sciences Division, School of Medicine, King's College London, London, UK.
  • 3 Laboratory of Molecular Nutrition, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: luozhi99@mail.hzau.edu.cn.
Abstract

At present, the harmful effects and relevant mechanism of oxidized fish oils on fish and fish cells remain unknown. Our study found that oxidized fish oils increased lipogenesis, and reduced lipolysis, activated oxidative stress by decreasing Glutathione Peroxidase (GPX) activity, increasing malondialdhyde (MDA) content and damaging mitochondrial structure, and activated Autophagy in the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our further investigation indicated that oxEPA-activated lipophagy was via inhibiting the DNA binding capacity of the cAMP-response element binding protein (CREB)-1 to the region of Bcl-2 promoter, which in turn suppressed the binding activity of Bcl-2 to Beclin1 and promoted autophagosome formation. For the first time, our study elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish.

Keywords

Lipid metabolism; Molecular mechanism; Oxidative stress; Oxidized fish oils; Signaling pathway.

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