2. Academic Validation
  3. Mitochondrial Membrane Potential Assay

Mitochondrial Membrane Potential Assay

  • Methods Mol Biol. 2022;2474:11-19. doi: 10.1007/978-1-0716-2213-1_2.
Srilatha Sakamuru 1,  Jinghua Zhao 1,  Matias S Attene-Ramos 2,  Menghang Xia 3
Affiliations

Affiliations

  • 1 National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.
  • 2 Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
  • 3 National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA. mxia@mail.nih.gov.
PMID: 35294751 DOI: 10.1007/978-1-0716-2213-1_2
Abstract

Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2, HepaRG, and AC16 cells. A homogenous cell-based MMP assay has been optimized and performed in a 1536-well plate format, which can be used to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.

Keywords

1536-well plate; Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP); Mitochondrial membrane potential (MMP); Mitochondrial membrane potential indicator (m-MPI); Mitochondrial toxicity.

Figures