2. Academic Validation
  3. SHIP164 is a chorein motif lipid transfer protein that controls endosome-Golgi membrane traffic

SHIP164 is a chorein motif lipid transfer protein that controls endosome-Golgi membrane traffic

  • J Cell Biol. 2022 Jun 6;221(6):e202111018. doi: 10.1083/jcb.202111018.
Michael G Hanna # 1 2 3 4,  Patreece H Suen # 2,  Yumei Wu 1 2 3 4,  Karin M Reinisch 2 5,  Pietro De Camilli 1 2 3 4 6 5
Affiliations

Affiliations

  • 1 Department of Neuroscience, Yale University School of Medicine, New Haven, CT.
  • 2 Department of Cell Biology, Yale University School of Medicine, New Haven, CT.
  • 3 Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT.
  • 4 Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT.
  • 5 Aligning Science Across Parkinson's Collaborative Research Network, Chevy Chase, MD.
  • 6 Kavli Institue for Neuroscience, Yale University School of Medicine, New Haven, CT.
  • # Contributed equally.
PMID: 35499567 DOI: 10.1083/jcb.202111018
Abstract

Cellular membranes differ in protein and lipid composition as well as in the protein-lipid ratio. Thus, progression of membranous organelles along traffic routes requires mechanisms to control bilayer lipid chemistry and their abundance relative to proteins. The recent structural and functional characterization of VPS13-family proteins has suggested a mechanism through which lipids can be transferred in bulk from one membrane to another at membrane contact sites, and thus independently of vesicular traffic. Here, we show that SHIP164 (UHRF1BP1L) shares structural and lipid transfer properties with these proteins and is localized on a subpopulation of vesicle clusters in the early endocytic pathway whose membrane cargo includes the cation-independent mannose-6-phosphate receptor (MPR). Loss of SHIP164 disrupts retrograde traffic of these organelles to the Golgi complex. Our findings raise the possibility that bulk transfer of lipids to endocytic membranes may play a role in their traffic.

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