2. Academic Validation
  3. Different effects of hydrogen-rich water intake and hydrogen gas inhalation on gut microbiome and plasma metabolites of rats in health status

Different effects of hydrogen-rich water intake and hydrogen gas inhalation on gut microbiome and plasma metabolites of rats in health status

  • Sci Rep. 2022 May 4;12(1):7231. doi: 10.1038/s41598-022-11091-1.
Fei Xie # 1 2,  Xue Jiang # 1 2,  Yang Yi # 1 2,  Zi-Jia Liu 1 2,  Chen Ma 1 2,  Jin He 1 2,  Zhi-Ming Xun 1 2,  Meng Wang 1 2,  Meng-Yu Liu 1 2,  Yao Mawulikplimi Adzavon 1 2,  Peng-Xiang Zhao 1 2,  Xue-Mei Ma 3 4
Affiliations

Affiliations

  • 1 Faculty of Environment and Life, Beijing University of Technology, No. 100, Pingleyuan, Chaoyang District, Beijing, 100124, China.
  • 2 Beijing Molecular Hydrogen Research Center, Beijing, 100124, China.
  • 3 Faculty of Environment and Life, Beijing University of Technology, No. 100, Pingleyuan, Chaoyang District, Beijing, 100124, China. xmma@bjut.edu.cn.
  • 4 Beijing Molecular Hydrogen Research Center, Beijing, 100124, China. xmma@bjut.edu.cn.
  • # Contributed equally.
PMID: 35508571 DOI: 10.1038/s41598-022-11091-1
Abstract

The potential for preventive and therapeutic applications of H2 have now been confirmed in various disease. However, the effects of H2 on health status have not been fully elucidated. Our previous study reported changes in the body weight and 13 serum biochemical parameters during the six-month hydrogen intervention. To obtain a more comprehensive understanding of the effects of long-term hydrogen consumption, the plasma metabolome and gut microbiota were investigated in this study. Compared with the control group, 14 and 10 differential metabolites (DMs) were identified in hydrogen-rich water (HRW) and hydrogen inhalation (HI) group, respectively. Pathway enrichment analysis showed that HRW intake mainly affected starch and sucrose metabolism, and DMs in HI group were mainly enriched in arginine biosynthesis. 16S rRNA gene Sequencing showed that HRW intake induced significant changes in the structure of gut microbiota, while no marked Bacterial community differences was observed in HI group. HRW intake mainly induced significant increase in the abundance of Lactobacillus, Ruminococcus, Clostridium XI, and decrease in Bacteroides. HI mainly induced decreased abundances of Blautia and Paraprevotella. The metabolic function was determined by metabolic cage analysis and showed that HI decreased the voluntary intake and excretions of rats, while HRW intake did not. The results of this study provide basic data for further research on hydrogen medicine. Determination of the effects of hydrogen intervention on microbiota profiles could also shed LIGHT on identification of mechanism underlying the biological effects of molecular hydrogen.

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