2. Academic Validation
  3. Marker-assisted pyramiding of γ-tocopherol methyltransferase and glutamate formiminotransferase genes for development of biofortified sweet corn hybrids

Marker-assisted pyramiding of γ-tocopherol methyltransferase and glutamate formiminotransferase genes for development of biofortified sweet corn hybrids

  • PeerJ. 2022 Jul 6:10:e13629. doi: 10.7717/peerj.13629.
Guihua Lv # 1,  Xiaolong Chen # 1,  Duo Ying 1,  Jiansheng Li 2,  Yinghu Fan 3,  Bin Wang 4,  Ruiqiu Fang 1
Affiliations

Affiliations

  • 1 Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, Zhejiang, China.
  • 2 National Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China.
  • 3 Chuxiong Academy of Agricultural Sciences, Chuxiong, China.
  • 4 Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
  • # Contributed equally.
PMID: 35818359 DOI: 10.7717/peerj.13629
Abstract

Micronutrients, including Vitamins, Minerals, and other bioactive compounds, have tremendous impacts on human health. Much progress has been made in improving the micronutrient content of inbred lines in various crops through biofortified breeding. However, biofortified breeding still falls short for the rapid generation of high-yielding hybrids rich in multiple micronutrients. Here, we bred multi-biofortified sweet corn hybrids efficiently through marker-assisted selection. Screening by molecular markers for vitamin E and folic acid, we obtained 15 inbred lines carrying favorable alleles (six for vitamin E, nine for folic acid, and three for both). Multiple biofortified corn hybrids were developed through crossing and genetic diversity analysis.

Keywords

Biofortification; Hybrids; Molecular breeding; Sweet corn.

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