1. Academic Validation
  2. OL-FS13 alleviates experimental cerebral ischemia-reperfusion injury

OL-FS13 alleviates experimental cerebral ischemia-reperfusion injury

  • Exp Neurol. 2022 Nov;357:114180. doi: 10.1016/j.expneurol.2022.114180.
Naixin Liu 1 Yilin Li 1 Ying Yang 2 Longjun Shu 3 Yixiang Liu 3 Yutong Wu 1 Dandan Sun 1 Zijian Kang 1 Yue Zhang 1 Dan Ni 1 Ziqi Wei 1 Shanshan Li 1 Meifeng Yang 1 Ying Wang 4 Jun Sun 5 Xinwang Yang 6
Affiliations

Affiliations

  • 1 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
  • 2 Endocrinology Department of affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China.
  • 3 Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China.
  • 4 Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China. Electronic address: wangying_814@163.com.
  • 5 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: sunjun6661@126.com.
  • 6 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: yangxinwanghp@163.com.
Abstract

Cerebral ischemia-reperfusion (I/R) is the main cause of neurological injury after stroke. However, existing treatments for I/R injury are relatively poor, and relevant drugs need to be further explored. Amphibians have received increasing attention as a resource bank of bioactive Peptides. However, reports on neuroprotective Peptides from amphibians remain extremely rare. Here, we identified a new neuroprotective peptide (OL-FS13, amino acid sequence: FSLLLTWWRRRVC) from the odorous frog species Odorrana livida using a constructed cDNA library. OL-FS13 significantly improving infarct volume, behavioral and histological abnormalities in rats, and also showed neuroprotective activities in PC12 cell (by oxygen glucose deprivation/reoxygenation, OGD/R). Mechanistically, OL-FS13 increased the level of antioxidative Enzymes to resist oxidative stress and alleviated endoplasmic reticulum (ER) stress induced by I/R and OGD/R. The use of ML385 (Nrf2 inhibitor) indicated that OL-FS13 relieved nerve damage caused by oxidative and ER stress by increasing the nuclear displacement of Nrf2. Collectively, this research provides a novel drug candidate for the clinical cerebral I/R curation.

Keywords

Bioactive peptide; Cerebral ischemia; Neuroprotection; Oxidative stress; Reperfusion.

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