2. Academic Validation
  3. Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe

Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe

  • Anal Chem. 2022 Aug 16;94(32):11216-11223. doi: 10.1021/acs.analchem.2c01691.
Lei Feng 1 2 3,  Ying Deng 2,  Shufan Song 2,  Yanqiu Sun 2,  Jingnan Cui 4,  Xiaochi Ma 1 2,  Lingling Jin 2,  Yan Wang 2,  Tony D James 3 5,  Chao Wang 1 2
Affiliations

Affiliations

  • 1 Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China.
  • 2 Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Pharmacy, College of Integrative Medicine, Dalian Medical University, Dalian 116044, China.
  • 3 School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
  • 4 State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • 5 Department of Chemistry, University of Bath, Bath BA2 7AY, U.K.
PMID: 35920602 DOI: 10.1021/acs.analchem.2c01691
Abstract

Lipase found in the gut microbiota participates in the digestion and absorption of dietary fats. As such, the gut microbiota is involved in the regulation of the host metabolism, affecting the levels of lipids and free fatty acids, ultimately resulting in obesity. In this study, an enzymatic activatable near-infrared fluorescent probe, DDAO-C6, was developed for visually sensing endogenous Lipase from gut microbes. Using DDAO-C6, a cultivated intestinal yeast strain was rapidly identified from human feces that exhibited high Lipase expression and was identified as Trichosporon asahii Y2. We then determined that the colonization of the gut of mice with T. asahii Y2 increased Lipase activity in the digestive tract and promoted obesity and hyperlipidemia when the mice were fed high fat diets. Above all, the present research resulted in a fluorescence visualization tool for the functional investigation of gut microbiota associated with obesity and disorders of lipid metabolism.

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