2. Academic Validation
  3. 12-OAHSA is a component of olive oil and mitigates obesity-induced inflammation

12-OAHSA is a component of olive oil and mitigates obesity-induced inflammation

  • J Nutr Biochem. 2022 Dec:110:109127. doi: 10.1016/j.jnutbio.2022.109127.
Knowledge Mudhibadhi Moyo 1,  Jieun Choi 2,  Jiyeon Chang 3,  Shindy Soedono 3,  Dan Vo Hoang Nguyet 3,  Yu-Ri Song 1,  Soo Jin Park 2,  Gwang-Woong Go 4,  Do Yup Lee 5,  Kae Won Cho 6
Affiliations

Affiliations

  • 1 Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of Korea.
  • 2 Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, CALS, Seoul National University, Seoul, Republic of Korea.
  • 3 Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of Korea.
  • 4 Department of Food and Nutrition, Hanyang University, Seoul, Republic of Korea.
  • 5 Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, CALS, Seoul National University, Seoul, Republic of Korea. Electronic address: rome73@snu.ac.kr.
  • 6 Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan, Republic of Korea; Department of Integrated Biomedical Science, Soonchunhyang University, Cheonan, Republic of Korea. Electronic address: kwcho@sch.ac.kr.
PMID: 35977667 DOI: 10.1016/j.jnutbio.2022.109127
Abstract

Fatty acid esters of hydroxyl fatty acids (FAHFAs) are a new family of endogenous lipids that exert anti-inflammatory action. Among the various FAHFA isomers, the dietary source of oleic acid-hydroxy stearic acid (OAHSA) and its anti-inflammatory functions are poorly understood. This study investigated the composition of OAHSA isomers in dietary oils and the impact of 12-OAHSA on obesity-induced inflammation. Liquid chromatography with tandem mass spectrometry analysis revealed that various dietary oils, including fish oil, corn oil, palm oil, soybean oil, and olive oil, present a wide variation in OAHSA profiles and amounts. The highest amounts of total OAHSAs are present in olive oil including 12-OAHSA. Compared to vehicle-treated obese mice, administration of 12-OAHSA significantly improved glucose homeostasis, independent of body weight. 12-OAHSA-treated mice displayed significantly reduced accumulation of CD11c+ adipose tissue macrophages, and CD4+/CD8+ adipose tissue T lymphocytes. Concomitantly, the expression of pro-inflammatory cytokine genes and the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway were significantly decreased in the 12-OAHSA-treated adipose tissue, while the expression of the anti-inflammatory gene Il10 was markedly increased. Moreover, in vitro Cell Culture experiments showed that 12-OAHSA significantly inhibited the lipopolysaccharides-induced inflammatory response in macrophages by suppressing the nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway. Collectively, these results indicated that 12-OAHSA, as a component of olive oil, mitigates obesity-induced Insulin resistance by regulating AT inflammation. Therefore, 12-OAHSA could be used as a novel nutritional intervention against obesity-associated metabolic dysregulation.

Keywords

12-OAHSA; Adipose tissue inflammation; FAHFA; LC-MS/MS; Olive oil.

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