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  2. Transcriptomic Analysis Reveals Lactobacillus reuteri Alleviating Alcohol-Induced Liver Injury in Mice by Enhancing the Farnesoid X Receptor Signaling Pathway

Transcriptomic Analysis Reveals Lactobacillus reuteri Alleviating Alcohol-Induced Liver Injury in Mice by Enhancing the Farnesoid X Receptor Signaling Pathway

  • J Agric Food Chem. 2022 Oct 5;70(39):12550-12564. doi: 10.1021/acs.jafc.2c05591.
Yonglang Cheng 1 Xin Xiang 2 Chen Liu 1 Tianying Cai 1 Tongxi Li 1 Yifan Chen 1 Junjie Bai 1 Hao Shi 1 Tianxiang Zheng 1 Meizhou Huang 3 Wenguang Fu 1 3
Affiliations

Affiliations

  • 1 Department of General Surgery (Hepatopancreatobiliary Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou646000, Sichuan, China.
  • 2 Department of General Surgery, The First People's Hospital of Neijiang, Neijiang641000, Sichuan, China.
  • 3 Academician (Expert) Workstation of Sichuan Province, The Affiliated Hospital of Southwest Medical University, Luzhou646000, Sichuan, China.
Abstract

Alcoholic liver disease (ALD) is caused by alcohol abuse and can progress to hepatitis, cirrhosis, and even hepatocellular carcinoma. Previous studies suggested that Lactobacillus reuteri (L. reuteri) ameliorates ALD, but the exact mechanisms are not fully known. This study created an ALD model in mice, and the results showed L. reuteri significantly alleviating lipid accumulation in the mice. Transcriptome Sequencing showed the L. reuteri treatment group had the most enriched metabolic pathway genes. We then studied the farnesoid X receptor (FXR) metabolic pathway in the mice liver tissue. Western blot analysis showed that FXR and carbohydrate response element binding protein (ChREBP) were upregulated and sterol regulatory element binding transcription factor 1 (Srebf1) and Cluster of differentiation (CD36) were downregulated in the L. reuteri-treated group. Subsequently, we administered FXR inhibitor glycine-β-muricholic acid (Gly-β-MCA) to mice, and the results show that Gly-β-MCA could reduce the therapeutic effect of L. ruteri. In conclusion, our study shows L. reuteri improved liver lipid accumulation in mice via the FXR signaling regulatory axis and may be a viable treatment option for ALD.

Keywords

Lactobacillus reuteri; alcohol; lipid metabolism; liver injury; transcriptome.

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