2. Academic Validation
  3. Activation of progesterone receptor is essential for folic acid-regulated cancer cell proliferation and migration

Activation of progesterone receptor is essential for folic acid-regulated cancer cell proliferation and migration

  • J Nutr Biochem. 2023 Feb:112:109205. doi: 10.1016/j.jnutbio.2022.109205.
Hui-Chen Wang 1,  Yen-Nien Huo 2,  Wen-Sen Lee 3
Affiliations

Affiliations

  • 1 Graduate Institutes of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan; Second Degree Bachelor of Science in Nursing, College of Medicine, National Taiwan University, Taipei, Taiwan.
  • 2 Graduate Institutes of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.
  • 3 Graduate Institutes of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan; Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan; Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Department of Physiology, School of Medicine, Tzu Chi University, Hualien, Taiwan. Electronic address: wslee@tmu.edu.tw.
PMID: 36455835 DOI: 10.1016/j.jnutbio.2022.109205
Abstract

We previously demonstrated that activation of Progesterone Receptor (PR) is essential for folic acid (FA)-inhibited proliferation in colorectal Cancer cell lines. In the present study, we further investigated whether the requirement of PR activation for the FA-regulated cell proliferation and migration is a general phenomenon for all Cancer cell lines or specific for colorectal Cancer cell lines only. Initially, we examined the expression of PR in various Cancer cell lines using Western blot analyses and RT-PCR technique, and then investigated the effects of FA on these Cancer cell lines. Our data showed that the effects of FA on proliferation and migration only occurred in the PR positive (+) Cancer cell lines, but not the PR negative (-) Cancer cell lines, and these effects were abolished by pre-treatment with the PR specific inhibitor, Org 31710. On the other hand, FA significantly reduced the proliferation and migration in the PR (-) Cancer cell lines transfected with PR pcDNA. However, FA did not significantly affect the proliferation and migration in the PR-transefected Hep-3B cell line, which does not express endogenous PR and FA receptor (FR). Since we previously showed that FA-regulated proliferation in colorectal and breast Cancer cell lines through the cSrc-mediated pathway, we conducted immunoprecipitation assay to demonstrate that PR formed a complex with FR and cSrc, but FR did not directly associate with cSrc. Taken together, these findings suggest that the requirement of PR activation for the FA-regulated cell proliferation and migration is a general phenomenon for all Cancer cell lines.

Keywords

Cancer cell lines; Migration; Overexpression; Progesterone receptor; Proliferation.

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