2. Academic Validation
  3. Emodin alleviates lung ischemia-reperfusion injury by suppressing gasdermin D-mediated pyroptosis in rats

Emodin alleviates lung ischemia-reperfusion injury by suppressing gasdermin D-mediated pyroptosis in rats

  • Clin Respir J. 2023 Feb 7. doi: 10.1111/crj.13582.
Tao Jin 1,  Fen Ai 2,  Jin Zhou 1,  Lin Kong 1,  Zhangming Xiong 1,  Dingping Wang 3,  Ruilin Lu 3,  Zhen Chen 2,  Muxi Zhang 4
Affiliations

Affiliations

  • 1 Department of Anesthesiology, Suining First People's Hospital, Suining, Sichuan, China.
  • 2 Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 3 Department of Proctology, Suining First People's Hospital, Suining, Sichuan, China.
  • 4 Department of Ophthalmology, Suining First People's Hospital, Suining, Sichuan, China.
PMID: 36751097 DOI: 10.1111/crj.13582
Abstract

Background: Pyroptosis refers to programmed cell death associated with inflammation. Emodin has been reported to alleviate lung injuries caused by various pathological processes and attenuate ischemia-reperfusion (I/R) injuries in diverse tissues.

Methods: Lewis rats were assigned into the sham, the I/R, and the I/R + emodin groups. Emodin and phosphate-buffered saline were intraperitoneally injected into rats of the emodin group and I/R group for 30 min, respectively. These rats were then subjected to left thoracotomy followed by 90-min clamping of the left hilum and 120-min reperfusion. Sham-operated rats underwent 210-min ventilation. Lung functions, histological changes, lung edema, and cytokine levels were assessed. Protein levels were measured by western blotting. Immunofluorescence staining was conducted to evaluate Pyroptosis.

Results: Emodin alleviated the I/R-induced lung dysfunction, lung damages, and inflammation. Protective effects of emodin against I/R-mediated endothelial Pyroptosis was observed in vivo and in vitro. Mechanistically, emodin inactivated the TLR4/MyD88/NF-κB/NLRP3 pathway.

Conclusion: Emodin attenuates lung ischemia-reperfusion injury by inhibiting GSDMD-mediated Pyroptosis in rats.

Keywords

NLRP3; emodin; gasdermin D; lung ischemia-reperfusion; pyroptosis.

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